ArticleBMC research notes2015
Derivation of induced pluripotent stem cells from orangutan skin fibroblasts.
Article in BMC research notes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Review
- Induction of pluripotency in large Japanese field mouse (Apodemus speciosus) fibroblasts via reprogramming.The Journal of veterinary medical science · 2026Article
- Application of induced pluripotent stem cells in the conservation of endangered animals.Stem cell research & therapy · 2025Review
- Generation and characterization of induced pluripotent stem cells of small apes.Frontiers in cell and developmental biology · 2025Article
- Inducing Pluripotency in Somatic Cells: Historical Perspective and Recent Advances.International journal of stem cells · 2024Review
- Advancing stem cell technologies for conservation of wildlife biodiversity.Development (Cambridge, England) · 2024Review
- The impact of induced pluripotent stem cells in animal conservation.Veterinary research communications · 2024Review
- Article
- Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species.Scientific reports · 2024Article
- Advances in Genetic Reprogramming: Prospects from Developmental Biology to Regenerative Medicine.Current medicinal chemistry · 2024Review
- An expedition in the jungle of pluripotent stem cells of non-human primates.Stem cell reports · 2023Review
- Generation of induced pluripotent stem cells from Bornean orangutans.Frontiers in cell and developmental biology · 2023Article
- Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking.Reproduction & fertility · 2022Review
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- Genetic Signatures of Evolution of the Pluripotency Gene Regulating Network across Mammals.Genome biology and evolution · 2020Article
- Reverse engineering human brain evolution using organoid models.Brain research · 2020Review
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
- Prospects for the Use of Induced Pluripotent Stem Cells in Animal Conservation and Environmental Protection.Stem cells translational medicine · 2019Article
- An ecotoxicological view on neurotoxicity assessment.Environmental sciences Europe · 2018Review
- Flexible adaptation of male germ cells from female iPSCs of endangeredScience advances · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundOrangutans are an endangered species whose natural habitats are restricted to the Southeast Asian islands of Borneo and Sumatra. Along with the African great apes, orangutans are among the closest living relatives to humans. For potential species conservation and functional genomics studies, we derived induced pluripotent stem cells (iPSCs) from cryopreserved somatic cells obtained from captive orangutans.
resultsPrimary skin fibroblasts from two Sumatran orangutans were transduced with retroviral vectors expressing the human OCT4, SOX2, KLF4, and c-MYC factors. Candidate orangutan iPSCs were characterized by global gene expression and DNA copy number analysis. All were consistent with pluripotency and provided no evidence of large genomic insertions or deletions. In addition, orangutan iPSCs were capable of producing cells derived from all three germ layers in vitro through embryoid body differentiation assays and in vivo through teratoma formation in immune-compromised mice.
conclusionsWe demonstrate that orangutan skin fibroblasts are capable of being reprogrammed into iPSCs with hallmark molecular signatures and differentiation potential. We suggest that reprogramming orangutan somatic cells in genome resource banks could provide new opportunities for advancing assisted reproductive technologies relevant for species conservation efforts. Furthermore, orangutan iPSCs could have applications for investigating the phenotypic relevance of genomic changes that occurred in the human, African great ape, and/or orangutan lineages. This provides opportunities for orangutan cell culture models that would otherwise be impossible to develop from living donors due to the invasive nature of the procedures required for obtaining primary cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.