ArticleParasites & vectors2015
Characterization and annotation of Babesia orientalis apicoplast genome.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists.PLoS pathogens · 2025Review
- Novel Strategy for In Vitro Validation of Babesia orientalis Heat Shock Proteins Chaperone Activity and Thermostability.Acta parasitologica · 2024Article
- Complete Genomes ofPathogens (Basel, Switzerland) · 2022Article
- Identification of a novel variant erythrocyte surface antigen-1 (VESA1) in Babesia orientalis.Parasitology research · 2021Article
- Capture-based enrichment of Theileria parva DNA enables full genome assembly of first buffalo-derived strain and reveals exceptional intra-specific genetic diversity.PLoS neglected tropical diseases · 2020Article
- Annotation and characterization of Babesia gibsoni apicoplast genome.Parasites & vectors · 2020Article
- There Is Treasure Everywhere: Reductive Plastid Evolution in Apicomplexa in Light of Their Close Relatives.Biomolecules · 2019Review
- Comparative analysis of apicoplast genomes of Babesia infective to small ruminants in China.Parasites & vectors · 2019Article
- Insights into the evolution and drug susceptibility of Babesia duncani from the sequence of its mitochondrial and apicoplast genomes.International journal for parasitology · 2019Article
- Comparative Analysis of Erythrocyte Proteomes of Water Buffalo, Dairy Cattle, and Beef Cattle by Shotgun LC-MS/MS.Frontiers in veterinary science · 2019Article
- The MEP pathway in Babesia orientalis apicoplast, a potential target for anti-babesiosis drug development.Parasites & vectors · 2018Article
- Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes.Parasites & vectors · 2018Article
- Reductive evolution of chloroplasts in non-photosynthetic plants, algae and protists.Current genetics · 2018Review
- Molecular Cloning and Characterization ofJournal of parasitology research · 2017Article
- A Historical Overview of Research onFrontiers in microbiology · 2017Review
- Comparative sequence analysis of Cyclospora cayetanensis apicoplast genomes originating from diverse geographical regions.Parasites & vectors · 2016Article
- A method for identification of highly conserved elements and evolutionary analysis of superphylum Alveolata.BMC bioinformatics · 2016Article
- Regulation of Expression and Evolution of Genes in Plastids of Rhodophytic Branch.Life (Basel, Switzerland) · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBabesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (Bubalus bubalis, Linnaeus, 1758) transmitted by the tick Rhipicephalus heamaphysaloides. It is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern China. As a member of the phylum Apicomplexa, B. orientalis possesses a relatively independent and alga originated organelle the apicoplast. Apicoplasts in other apicomplexa parasites are involved in the biosynthesis of haem, fatty acids, iron-sulphur clusters and isoprenoids. Some of these metabolic pathways were shown to be essential for parasite survival, therefore can serve as potential drug targets.
methods30 pairs of primers were designed based on the full genome sequence of B. orientalis (unpublished data) and by aligning reported apicoplast genomes of Babesia bovis and Theileria parva. Conventional PCRs was performed to obtain overlapped fragments to cover the whole apicoplast genome. Then the apicoplast genome of B.orientalis was sequenced, assembled and aligned with reported apicoplast genomes of B. bovis and T. parva. The obtained apicoplast genome was annotated by using Artemis and comparing with published apicomplexan apicoplast genomes. The SSU and LSU nucleotide sequences generated were used in a phylogenetic analysis using the maximum likelihood implemented in MAGE 6.0.
resultsWe have obtained and analyzed the complete genome sequence of the B. orientalis apicoplast. It consisted of a 33.2 kb circular DNA (78.9 % A + T). The apicoplast genome unidirectionally encodes one large and one small subunit ribosomal RNAs, 24 tRNA genes, 4 DNA-dependent RNA polymerase beta subunits (rpoB, rpoC1, rpoC2a and rpoC2b), 17 ribosomal proteins, one EF-Tu elongation factor, 2 Clp protease chaperones, and 14 hypothetical proteins. In addition, it includes two copies of the clpC gene. The structure and organization of the B. orientalis apicoplast genome are most similar to those of the B. bovis apicoplast.
conclusionsThis is the first report of the complete sequence of the B. orientalis apicoplast genome. This information should be useful in the development of safe and efficient treatment against buffalo babesiosis.
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