ArticleBMC microbiology2015
Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Design and validation of an inducible and curable EvolvR system for directed evolution inFrontiers in bioengineering and biotechnology · 2026Article
- Integrated genomic and transcriptomic Insights into methanol tolerance mechanisms in Methylobacterium extorquens AM1, identifying key targets for strain engineering.Journal of biological engineering · 2025Article
- Metabolic engineering for microbial production of sugar acids.BMC biotechnology · 2025Review
- Engineering of the DNA replication and repair machinery to develop binary mutators for rapid genome evolution of Corynebacterium glutamicum.Nucleic acids research · 2023Article
- A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms.NPJ biofilms and microbiomes · 2023Article
- From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles.Microbial cell factories · 2023Article
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- Developing Synthetic Methylotrophs by Metabolic Engineering-Guided Adaptive Laboratory Evolution.Advances in biochemical engineering/biotechnology · 2022Article
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum.Microbial cell factories · 2021Article
- Evolving a New Efficient Mode of Fructose Utilization for Improved Bioproduction inFrontiers in bioengineering and biotechnology · 2021Article
- Improving the Methanol Tolerance of anFrontiers in microbiology · 2021Article
- Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris.BMC microbiology · 2020Article
- Novel outlook in engineering synthetic methylotrophs and formatotrophs: a course for advancing C1-based chemicals production.World journal of microbiology & biotechnology · 2020Review
- FermentativeMicroorganisms · 2020Article
- Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum.Communications biology · 2020Article
- Recent Progress on Chemical Production From Non-food Renewable Feedstocks UsingFrontiers in bioengineering and biotechnology · 2020Review
- Continuous Culture Adaptation ofFrontiers in microbiology · 2019Article
- Bromination of L-tryptophan in a Fermentative Process WithFrontiers in bioengineering and biotechnology · 2019Article
- Efficient Production of the Dicarboxylic Acid Glutarate byFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMethanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes.
resultsThe methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by experimental evolution in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde and formate was not improved. Genome sequence analysis of strain Tol1 revealed two point mutations potentially relevant to enhanced methanol tolerance: one leading to the amino acid exchange A165T of O-acetylhomoserine sulfhydrolase MetY and the other leading to shortened CoA transferase Cat (Q342*). Introduction of either mutation into the genome of C. glutamicum wild type increased methanol tolerance and introduction of both mutations into C. glutamicum was sufficient to achieve methanol tolerance almost indistinguishable from that of strain Tol1.
conclusionThe methanol tolerance of C. glutamicum can be increased by two point mutations leading to amino acid exchange of O-acetylhomoserine sulfhydrolase MetY and shortened CoA transferase Cat. Introduction of these mutations into producer strains may be helpful when using carbon sources containing methanol as component or impurity.
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