Evidence map›Paper›PMID 26474846›Full record

ArticleMolecular biology and evolution2016

An Exon-Capture System for the Entire Class Ophiuroidea.

Andrew F Hugall, Timothy D O'Hara, Sumitha Hunjan, Roger Nilsen, Adnan Moussalli

Abstract read
In one paragraph

Article in Molecular biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

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  20. Assexon: Assembling Exon Using Gene Capture Data.Evolutionary bioinformatics online · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Andrew F HugallMuseum Victoria, Melbourne, Vic., Australia ahugall@museum.vic.gov.au tohara@museum.vic.gov.au.
Timothy D O'HaraMuseum Victoria, Melbourne, Vic., Australia ahugall@museum.vic.gov.au tohara@museum.vic.gov.au.
Sumitha HunjanMuseum Victoria, Melbourne, Vic., Australia.
Roger NilsenGeorgia Genomics Facility, University of Georgia.
Adnan MoussalliMuseum Victoria, Melbourne, Vic., Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exon-capture studies have typically been restricted to relatively shallow phylogenetic scales due primarily to hybridization constraints. Here, we present an exon-capture system for an entire class of marine invertebrates, the Ophiuroidea, built upon a phylogenetically diverse transcriptome foundation. The system captures approximately 90% of the 1,552 exon target, across all major lineages of the quarter-billion-year-old extant crown group. Key features of our system are 1) basing the target on an alignment of orthologous genes determined from 52 transcriptomes spanning the phylogenetic diversity and trimmed to remove anything difficult to capture, map, or align; 2) use of multiple artificial representatives based on ancestral state reconstructions rather than exemplars to improve capture and mapping of the target; 3) mapping reads to a multi-reference alignment; and 4) using patterns of site polymorphism to distinguish among paralogy, polyploidy, allelic differences, and sample contamination. The resulting data give a well-resolved tree (currently standing at 417 samples, 275,352 sites, 91% data-complete) that will transform our understanding of ophiuroid evolution and biogeography.

Indexed as

AnimalsDNA, MitochondrialEchinodermataExonsGenomicsPhylogenyDNA, MitochondrialEchinodermatahybrid enrichmentphylogenomics

Identifiers

PMID26474846
PMCPMC4693979

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.