ArticlePlant physiology2015
The CBL-Interacting Protein Kinase CIPK23 Regulates HAK5-Mediated High-Affinity K+ Uptake in Arabidopsis Roots.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.
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121citing papers in PubMed
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121 citing papers in PubMed, 233 citations in OpenAlex.
- The saltmarsh cordgrass Spartina anglica has novel genomic features that contribute to abiotic stress tolerance.Journal of experimental botany · 2026Article
- Identification of the HAK/KUP/KT Potassium Transporter Gene Family in Sweet Potato and Functional Characterization of IbHAK5A.Plants (Basel, Switzerland) · 2026Article
- Plant-Microbe Interactions in Sorghum Nutrient Acquisition: Roles of Rhizosphere Microbes and N, P, and K Transporters.Plants (Basel, Switzerland) · 2026Review
- The VvSAP8-VvHAK5 module from grape confers dual tolerance to potassium deficiency and salt stress by regulating potassium transport.Plant cell reports · 2026Article
- PPSR1 Protein Functions as an Important Regulator to Enhance Plant Growth Performance Under N, P, and K Deficient Stress Conditions.Plant biotechnology journal · 2026Article
- Molecular mechanisms and evolutionary adaptations of transporters for photosynthates and specialized metabolites in plants.Plant cell reports · 2026Review
- Transcriptome and weighted gene co-enrichment analysis revealed modules and candidate genes associated with barley response to low potassium stress.Frontiers in plant science · 2026Article
- Calcium signaling-mediated phosphorylation controls zinc allocation in Arabidopsis.Stress biology · 2025Article
- Vacuolar K⁺ efflux transporter TaTPK1-5D confers low-K⁺ tolerance of 'Zhengmai 136' through interaction with TaCIPK23-4D in bread wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Advances in deciphering the mechanisms of salt tolerance in Maize.Plant signaling & behavior · 2025Review
- SC35-mediated bZIP49 splicing regulates K⁺ channel AKT1 for salt stress adaptation in poplar.Nature communications · 2025Article
- Natural variation in potassium deficiency responses among Arabidopsis thaliana accessions.Plant & cell physiology · 2025Article
- Genome-Wide Identification and Expression Analysis of thePlants (Basel, Switzerland) · 2025Article
- Integration of GWAS and transcriptome approaches for the identification of nitrogen-, phosphorus-, and potassium-responsive genes in tomato.Horticulture research · 2025Article
- The high-affinity KTransgenic research · 2025Article
- The Potassium Utilization Gene Network inInternational journal of molecular sciences · 2025Article
- Article
- Identification, classification, and stress-responsive regulation ofFrontiers in plant science · 2025Article
- Salinity survival: molecular mechanisms and adaptive strategies in plants.Frontiers in plant science · 2025Review
- The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco.Plant signaling & behavior · 2024Article
61 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 4 institutions in 2 countries.
Paula RagelDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Reyes RódenasDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Elena García-MartínDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Zaida AndrésDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Irene VillaltaDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Manuel Nieves-CordonesDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Rosa M RiveroDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).ORCID http://orcid.org/0000-0003-3880-0241
Vicente MartínezDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).
Jose M PardoDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.).ORCID http://orcid.org/0000-0003-4510-8624
Francisco J QuinteroDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.) fjquintero@irnase.csic.es frubio@cebas.csic.es.ORCID http://orcid.org/0000-0001-8718-2975
Francisco RubioDepartamento de Biotecnología Vegetal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla E-41012, Spain (P.R., E.G.-M., Z.A., I.V., J.M.P., F.J.Q.);Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus de Espinardo, Murcia 30100, Spain (R.R., R.M.R., V.M., F.R.); andBiochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, Unité Mixte de Recherche 5004 Centre National de la Recherche Scientifique/Unité Mixte de Recherche 0386 Institut National de la Recherche Agronomique/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier cedex 2, France (M.N.-C.) fjquintero@irnase.csic.es frubio@cebas.csic.es.ORCID http://orcid.org/0000-0001-7640-9548
Consejo Superior de Investigaciones Científicas · ESInstituto de Productos Naturales y Agrobiología · ESCentro de Edafología y Biología Aplicada del Segura · ESInstitut des Sciences des Plantes de Montpellier · FR
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant growth and development requires efficient acquisition of essential elements. Potassium (K(+)) is an important macronutrient present in the soil solution at a wide range of concentrations. Regulation of the K(+) uptake systems in the roots is essential to secure K(+) supply. It has been shown in Arabidopsis (Arabidopsis thaliana) that when the external K(+) concentration is very low (<10 µm), K(+) nutrition depends exclusively on the high-affinity K(+) transporter5 (HAK5). Low-K(+)-induced transcriptional activation of the gene encoding HAK5 has been previously reported. Here, we show the posttranscriptional regulation of HAK5 transport activity by phosphorylation. Expression in a heterologous system showed that the Ca(2+) sensors calcineurin B-like (CBL1), CBL8, CBL9, and CBL10, together with CBL-interacting protein kinase23 (CIPK23), activated HAK5 in vivo. This activation produced an increase in the affinity and the Vmax of K(+) transport. In vitro experiments show that the N terminus of HAK5 is phosphorylated by CIPK23. This supports the idea that phosphorylation of HAK5 induces a conformational change that increases its affinity for K(+). Experiments of K(+) (Rb(+)) uptake and growth measurements in low-K(+) medium with Arabidopsis single mutants hak5, akt1, and cipk23, double mutants hak5 akt1, hak5 cipk23, and akt1 cipk23, and the triple mutant hak5 akt1 cipk23 confirmed the regulatory role of CIPK23 in planta.
Indexed as
ArabidopsisArabidopsis ProteinsBinding, CompetitiveCalcium-Binding ProteinsIon TransportKineticsMutationPhosphorylationPlant RootsPotassiumPotassium-Hydrogen AntiportersProtein BindingProtein Serine-Threonine KinasesRubidiumTwo-Hybrid System TechniquesArabidopsis Proteinscalcineurin B-like protein 1, ArabidopsisCalcium-Binding ProteinsCBL10 protein, ArabidopsisCBL9 protein, ArabidopsisCIPK23 protein, ArabidopsisPotassiumPotassium-Hydrogen Antiporterspotassium transporter, ArabidopsisProtein Serine-Threonine KinasesRubidium
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