ArticlePloS one2015
MiRNA Analysis by Quantitative PCR in Preterm Human Breast Milk Reveals Daily Fluctuations of hsa-miR-16-5p.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Non-Coding RNAs in Human Breast Milk: A Systematic Review.Frontiers in immunology · 2021Pooled it
- Pooled it
- Characterizing Human Milk RNA Degradation Over Time to Optimize Storage of Human Milk for RNA Quality.Journal of mammary gland biology and neoplasia · 2026Article
- Differential microRNAs and metabolites in the breast milk of mothers with adverse childhood experiences.Translational psychiatry · 2025Article
- Differential Expressions of Immunoregulatory microRNAs in Breast Milk of Mothers of Preterm Versus Term Infants: A Preliminary Study.Medicina (Kaunas, Lithuania) · 2025Article
- A review on cell-free RNA profiling: Insights into metabolic diseases and predictive value for bariatric surgery outcomes.Molecular metabolism · 2024Review
- Gestational age at birth influences protein and RNA content in human milk extracellular vesicles.Journal of extracellular biology · 2024Article
- Human Breast Milk miRNAs: Their Diversity and Potential for Preventive Strategies in Nutritional Therapy.International journal of molecular sciences · 2023Review
- Circadian Rhythm Does Not Affect the miRNA Cargo of Bovine Raw Milk Extracellular Vesicles.International journal of molecular sciences · 2023Article
- Occurrence, Role, and Challenges of MicroRNA in Human Breast Milk: A Scoping Review.Biomedicines · 2023Article
- Milk Exosomal microRNAs: Postnatal Promoters of β Cell Proliferation but Potential Inducers of β Cell De-Differentiation in Adult Life.International journal of molecular sciences · 2022Review
- Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula.Biotech (Basel (Switzerland)) · 2022Article
- Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.BMC genomics · 2022Article
- Melatonin in Early Nutrition: Long-Term Effects on Cardiovascular System.International journal of molecular sciences · 2021Review
- Review of Methodological Approaches to Human Milk Small Extracellular Vesicle Proteomics.Biomolecules · 2021Review
- Levels of Breast Milk MicroRNAs and Other Non-Coding RNAs Are Impacted by Milk Maturity and Maternal Diet.Frontiers in immunology · 2021Article
- Xeno-miRNA in Maternal-Infant Immune Crosstalk: An Aid to Disease Alleviation.Frontiers in immunology · 2020Review
- Characterization of Holstein and Normande whole milk miRNomes highlights breed specificities.Scientific reports · 2019Article
- Identification of miR-328-3p as an endogenous reference gene for the normalization of miRNA expression data from patients with Diabetic Retinopathy.Scientific reports · 2019Article
- Bta-miR-34b regulates milk fat biosynthesis by targeting mRNA decapping enzyme 1A (DCP1A) in cultured bovine mammary epithelial cells1.Journal of animal science · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsHuman breast milk is an extremely dynamic fluid containing many biologically-active components which change throughout the feeding period and throughout the day. We designed a miRNA assay on minimized amounts of raw milk obtained from mothers of preterm infants. We investigated changes in miRNA expression within month 2 of lactation and then over the course of 24 hours. MATERIALS AND
methodsAnalyses were performed on pooled breast milk, made by combining samples collected at different clock times from the same mother donor, along with time series collected over 24 hours from four unsynchronized mothers. Whole milk, lipids or skim milk fractions were processed and analyzed by qPCR. We measured hsa-miR-16-5p, hsa-miR-21-5p, hsa-miR-146-5p, and hsa-let-7a, d and g (all -5p). Stability of miRNA endogenous controls was evaluated using RefFinder, a web tool integrating geNorm, Normfinder, BestKeeper and the comparative ΔΔCt method.
resultsMiR-21 and miR-16 were stably expressed in whole milk collected within month 2 of lactation from four mothers. Analysis of lipids and skim milk revealed that miR-146b and let-7d were better references in both fractions. Time series (5H-23H) allowed the identification of a set of three endogenous reference genes (hsa-let-7d, hsa-let-7g and miR-146b) to normalize raw quantification cycle (Cq) data. We identified a daily oscillation of miR-16-5p. PERSPECTIVES: Our assay allows exploring miRNA levels of breast milk from mother with preterm baby collected in time series over 48-72 hours.
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Registered trials
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