ArticleACS synthetic biology2016
Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide.
Article in ACS synthetic biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- An ultralow-background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain.Nature methods · 2026Article
- Optogenetic control of PLC-γ1 activity directs cell motility.The Journal of cell biology · 2026Article
- Design and Implementation of a Blue-Light-Controlled Gene-Switch System.Molecules (Basel, Switzerland) · 2026Article
- Time-Resolved Native Mass Spectrometry Reveals Reversible Light-Driven Oligomerization of Arabidopsis Cryptochrome 1 and Its Antagonism by BIC1.Angewandte Chemie (International ed. in English) · 2026Article
- Article
- Resolving oligomeric states of photoactivatable proteins in living cells via photon counting histogram analysis.iScience · 2025Article
- A Modular Platform for the Optogenetic Control of Small GTPase Activity in Living Cells Reveals Long-Range RhoA Signaling.bioRxiv : the preprint server for biology · 2025Article
- Optogenetic control of PLC-γ1 activity polarizes cell motility.bioRxiv : the preprint server for biology · 2025Article
- Methods for Controlling Small GTPase Activity.Chembiochem : a European journal of chemical biology · 2025Review
- Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems.Synthetic and systems biotechnology · 2025Review
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- Analysis of Light-Controlled Artificial Cell-Cell Adhesions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Light-dependent modulation of protein localization and function in living bacteria cells.Nature communications · 2024Article
- Genetically-stable engineered optogenetic gene switches modulate spatial cell morphogenesis in two- and three-dimensional tissue cultures.Nature communications · 2024Article
- 'Seeing' the electromagnetic spectrum: spotlight on the cryptochrome photocycle.Frontiers in plant science · 2024Review
- High-Throughput Optogenetics Experiments in Yeast Using the Automated Platform Lustro.Journal of visualized experiments : JoVE · 2023Article
- Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit.ACS synthetic biology · 2023Article
- Spatio-temporal patterning of extensile active stresses in microtubule-based active fluids.PNAS nexus · 2023Article
- Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s.Nature methods · 2023Article
- Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells.Cell structure and function · 2023Article
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Authors and funding
5 authors.
Funding
Abstract
Light-inducible dimers are powerful tools for cellular optogenetics, as they can be used to control the localization and activity of proteins with high spatial and temporal resolution. Despite the generality of the approach, application of light-inducible dimers is not always straightforward, as it is frequently necessary to test alternative dimer systems and fusion strategies before the desired biological activity is achieved. This process is further hindered by an incomplete understanding of the biophysical/biochemical mechanisms by which available dimers behave and how this correlates to in vivo function. To better inform the engineering process, we examined the biophysical and biochemical properties of three blue-light-inducible dimer variants (cryptochrome2 (CRY2)/CIB1, iLID/SspB, and LOVpep/ePDZb) and correlated these characteristics to in vivo colocalization and functional assays. We find that the switches vary dramatically in their dark and lit state binding affinities and that these affinities correlate with activity changes in a variety of in vivo assays, including transcription control, intracellular localization studies, and control of GTPase signaling. Additionally, for CRY2, we observe that light-induced changes in homo-oligomerization can have significant effects on activity that are sensitive to alternative fusion strategies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.