ArticleClinical and experimental reproductive medicine2015
The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study.
Article in Clinical and experimental reproductive medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Effects of different seasons on the outcome ofFrontiers in reproductive health · 2026Article
- The mechanisms of energy balance between oxidative phosphorylation and glycolysis in human ovarian granulosa cells.Frontiers in physiology · 2026Article
- Article
- Mechanisms of Granulosa Cell Programmed Cell Death and Follicular Atresia in Polycystic Ovary Syndrome.Physiological research · 2025Review
- Transcriptional insights on the incomplete cytoplasmic maturation and developmental potential of oocytes cultured without granulosa cells in mice.BMC genomics · 2025Article
- Vitamin C Alleviates the Negative Effects of Heat Stress on Reproductive Processes by Regulating Amino Acid Metabolism in Granulosa Cells.Antioxidants (Basel, Switzerland) · 2024Article
- Human-induced pluripotent stem cell-derived ovarian support cell co-culture improves oocyte maturation in vitro after abbreviated gonadotropin stimulation.Human reproduction (Oxford, England) · 2023Article
- Ovarian tissue cryopreservation and transplantation: a review on reactive oxygen species generation and antioxidant therapy.Cell and tissue research · 2023Review
- Evaluation of endometrioma size effect on ovarian reserve, embryo quality and pregnancy outcome after in vitro fertilization cycle; a cross-sectional study.BMC women's health · 2023Article
- LncGSAR Controls Ovarian Granulosa Cell Steroidogenesis via Sponging MiR-125b to Activate SCAP/SREBP Pathway.International journal of molecular sciences · 2022Article
- Granulosa Cell Conditioned Medium Enhances The Rate of Mouse Oocyte In Vitro Maturation and Embryo Formation.Cell journal · 2022Article
- Article
- Long Non-Coding RNAInternational journal of molecular sciences · 2022Article
- Article
- Sequential IVM by CNP preincubation and cooperating of PGE2 with AREG enhances developmental competence of SCNT reconstructs in goat.Scientific reports · 2022Article
- Investigation of Metabolome Underlying the Biological Mechanisms of Acute Heat Stressed Granulosa Cells.International journal of molecular sciences · 2022Article
- Granulosa Cells Improved Mare Oocyte Cytoplasmic Maturation by Providing Collagens.Frontiers in cell and developmental biology · 2022Article
- Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep.PloS one · 2020Article
- Could fertility clinics offer a sizable improvement of live birth rates by maturing post-GVBD oocytes in vitro?Journal of assisted reproduction and genetics · 2019Article
- OocyteTurkish journal of obstetrics and gynecology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveIn order to increase the number of mature oocytes usable for intracytoplasmic sperm injection (ICSI), we aimed to investigate the effect of co-culturing granulosa cells (GCs) on human oocyte maturation in vitro, the fertilization rate, and embryo development.
methodsA total of 133 immature oocytes were retrieved and were randomly divided into two groups; oocytes that were cultured with GCs (group A) and oocytes that were cultured without GCs (group B). After in vitro maturation, only oocytes that displayed metaphase II (MII) underwent the ICSI procedure. The maturation and fertilization rates were analyzed, as well as the frequency of embryo development.
resultsThe mean age of the patients, their basal levels of follicle-stimulating hormone, and the number of oocytes recovered from the patients were all comparable between the two study groups. The number of oocytes that reached MII (mature oocytes) was 59 out of 70 (84.28%) in group A, compared to 41 out of 63 (65.07%) in group B (p=0.011). No significant difference between fertilization rates was found between the two study groups (p=0.702). The embryo development rate was higher in group A (33/59, 75%) than in group B (12/41, 42.85%; p=0.006). The proportion of highest-quality embryos and the blastocyst formation rate were significantly lower in group B than in group A (p=0.003 and p<0.001, respectively).
conclusionThe findings of the current study demonstrate that culturing immature human oocytes with GCs prior to ICSI improves the maturation rate and the likelihood of embryo development.
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