ArticlePlant methods2015
Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana.
Article in Plant methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- A rapid and efficient in planta assay for assessment of RNA editing factor performance.The Plant journal : for cell and molecular biology · 2026Article
- A quantitative split firefly luciferase complementation assay (SplitLUC) for in planta protein-protein interactions.Protoplasma · 2026Article
- Controlling heterologous protein synthesis through a plant RNA ThermoSwitch.Plant methods · 2026Article
- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
- A Scots pine 4-coumarate:CoA ligase with high cinnamic acid affinity is the primary candidate for pinosylvin biosynthesis.The Plant journal : for cell and molecular biology · 2026Article
- Transient Overexpression of the PepperLife (Basel, Switzerland) · 2025Article
- Critical parameters for robustPlant direct · 2024Article
- Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins.Journal of experimental botany · 2024Article
- Pineapple SWEET10 is a glucose transporter.Horticulture research · 2023Article
- Synthetic developmental biology: molecular tools to re-design plant shoots and roots.Journal of experimental botany · 2023Review
- Physiological and sucrose metabolic responses to waterlogging stress in balloon flower (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- RcbHLH59-RcPRs module enhances salinity stress tolerance by balancing NaHorticulture research · 2023Article
- Recruitment of distinct UDP-glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér.The New phytologist · 2023Article
- Petunia dihydroflavonol 4-reductase is only a few amino acids away from producing orange pelargonidin-based anthocyanins.Frontiers in plant science · 2023Article
- CRISPR-based resistance to grapevine virus A.Frontiers in plant science · 2023Article
- Generation of multi-layered protein bodies inFrontiers in plant science · 2023Article
- Polyketide reductases in defense-related parasorboside biosynthesis in Gerbera hybrida share processing strategies with microbial polyketide synthase systems.The New phytologist · 2022Article
- Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA-sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco.Plant cell reports · 2022Article
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Biological Phase Separation and Biomolecular Condensates in Plants.Annual review of plant biology · 2021Review
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3 authors.
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Abstract
backgroundTransient gene expression utilizing syringe agroinfiltration offers a simple and efficient technique for different transgenic applications. Leaves of Nicotiana benthamiana show reliable and high transformation efficiency, but in quantitative assays also a certain degree of variation. We used a nested design in our agroinfiltration experiments to dissect the sources of this variation.
resultsAn intron containing firefly luciferase gene was used as a reporter for agroinfiltration. A number of 6 week old tobacco plants were infiltrated for their top leaves, several samples were punched from the leaves after 2 days of transient expression, and protein extracts from the samples were repeatedly measured for luciferase activity. Interestingly, most of the variation was due to differences between the sampling spots in the leaves, the next important source being the different leaves on each plant. Variation between similar experiments, between plants and between repetitive measurements of the extracts could be easily minimized.
conclusionsEfforts and expenditure of agroinfiltration experiments can be optimized when sources of variation are known. In summary, infiltrate more plants but less leaves, sample more positions on the leaf but run only few technical replicates.
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