ArticleThe Journal of biological chemistry2015
ATP-binding Cassette (ABC) Transport System Solute-binding Protein-guided Identification of Novel d-Altritol and Galactitol Catabolic Pathways in Agrobacterium tumefaciens C58.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Activating the d-Tagatose Production Capacity ofJournal of agricultural and food chemistry · 2025Article
- Harnessing enzyme promiscuity of alditol-2-dehydrogenases for oxidation of alditols to enantiopure ketoses.PloS one · 2025Article
- Article
- SdiA Enhanced the Drug Resistance ofFrontiers in microbiology · 2022Article
- A Comprehensive Phylogenetic Analysis of the Serpin Superfamily.Molecular biology and evolution · 2021Article
- The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways.Biochemistry · 2019Article
- Galactitol catabolism in Sinorhizobium meliloti is dependent on a chromosomally encoded sorbitol dehydrogenase and a pSymB-encoded operon necessary for tagatose catabolism.Molecular genetics and genomics : MGG · 2019Article
- 'Democratized' genomic enzymology web tools for functional assignment.Current opinion in chemical biology · 2018Review
- Functional assignment of multiple catabolic pathways for D-apiose.Nature chemical biology · 2018Article
- Genomic Enzymology: Web Tools for Leveraging Protein Family Sequence-Function Space and Genome Context to Discover Novel Functions.Biochemistry · 2017Review
- Transport capabilities of environmental Pseudomonads for sulfur compounds.Protein science : a publication of the Protein Society · 2017Article
- Methanobactin transport machinery.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Assignment of function to a domain of unknown function: DUF1537 is a new kinase family in catabolic pathways for acid sugars.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.Acta crystallographica. Section F, Structural biology communications · 2016Article
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Authors and funding
7 authors.
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Abstract
Innovations in the discovery of the functions of uncharacterized proteins/enzymes have become increasingly important as advances in sequencing technology flood protein databases with an exponentially growing number of open reading frames. This study documents one such innovation developed by the Enzyme Function Initiative (EFI; U54GM093342), the use of solute-binding proteins for transport systems to identify novel metabolic pathways. In a previous study, this strategy was applied to the tripartite ATP-independent periplasmic transporters. Here, we apply this strategy to the ATP-binding cassette transporters and report the discovery of novel catabolic pathways for d-altritol and galactitol in Agrobacterium tumefaciens C58. These efforts resulted in the description of three novel enzymatic reactions as follows: 1) oxidation of d-altritol to d-tagatose via a dehydrogenase in Pfam family PF00107, a previously unknown reaction; 2) phosphorylation of d-tagatose to d-tagatose 6-phosphate via a kinase in Pfam family PF00294, a previously orphan EC number; and 3) epimerization of d-tagatose 6-phosphate C-4 to d-fructose 6-phosphate via a member of Pfam family PF08013, another previously unknown reaction. The epimerization reaction catalyzed by a member of PF08013 is especially noteworthy, because the functions of members of PF08013 have been unknown. These discoveries were assisted by the following two synergistic bioinformatics web tools made available by the Enzyme Function Initiative: the EFI-Enzyme Similarity Tool and the EFI-Genome Neighborhood Tool.
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