Evidence map›Paper›PMID 26472821›Full record

ArticleNeuro-oncology2016

TNF-α enhancement of CD62E mediates adhesion of non-small cell lung cancer cells to brain endothelium via CD15 in lung-brain metastasis.

Samah A Jassam, Zaynah Maherally, James R Smith, Keyoumars Ashkan, Federico Roncaroli, Helen L Fillmore, Geoffrey J Pilkington

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 35 citations in OpenAlex.

  1. Review
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  8. MicroRNAs in Lung Cancer Brain Metastasis.International journal of molecular sciences · 2024
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  9. The origin of brain malignancies at the blood-brain barrier.Cellular and molecular life sciences : CMLS · 2023
    Review
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  11. β-Estradiol 17-acetate enhances theNeural regeneration research · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Samah A JassamCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
Zaynah MaherallyCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
James R SmithCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
Keyoumars AshkanCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
Federico RoncaroliCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
Helen L FillmoreCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
Geoffrey J PilkingtonCellular and Molecular Neuro-oncology Research Group, Brain Tumour Research Centre, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK (S.A.J., Z.M., J.R.S., H.L.F., G.J.P.); Neuro-surgery, King's College Hospital, Denmark Hill, London, UK (K.A.); Institute of Brain Behaviour and Mental Health, The University of Manchester, Manchester, UK (F.R.).
Brain Tumour Research · GBUniversity of Portsmouth · GBKing's College Hospital · GBUniversity of Manchester · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCD15, which is overexpressed on various cancers, has been reported as a cell adhesion molecule that plays a key role in non-CNS metastasis. However, the role of CD15 in brain metastasis is largely unexplored. This study provides a better understanding of CD15/CD62E interaction, enhanced by tumor necrosis factor-α (TNF-α), and its correlation with brain metastasis in non-small cell lung cancer (NSCLC).

methodsCD15 and E-selectin (CD62E) expression was demonstrated in both human primary and metastatic NSCLC cells using flow cytometry, immunofluorescence, and Western blotting. The role of CD15 was investigated using an adhesion assay under static and physiological flow live-cell conditions. Human tissue sections were examined using immunohistochemistry.

resultsCD15, which was weakly expressed on hCMEC/D3 human brain endothelial cells, was expressed at high levels on metastatic NSCLC cells (NCI-H1299, SEBTA-001, and SEBTA-005) and at lower levels on primary NSCLC (COR-L105 and A549) cells (P < .001). The highest expression of CD62E was observed on hCMEC/D3 cells activated with TNF-α, with lower levels on metastatic NSCLC cells followed by primary NSCLC cells. Metastatic NSCLC cells adhered most strongly to hCMEC/D3 compared with primary NSCLC cells. CD15 immunoblocking decreased cancer cell adhesion to brain endothelium under static and shear stress conditions (P < .0001), confirming a correlation between CD15 and cerebral metastasis. Both CD15 and CD62E expression were detected in lung metastatic brain biopsies.

conclusionThis study enhances the understanding of cancer cell-brain endothelial adhesion and confirms that CD15 plays a crucial role in adhesion in concert with TNF-α activation of its binding partner, CD62E.

Indexed as

Blotting, WesternBrain NeoplasmsCarcinoma, Non-Small-Cell LungCell AdhesionCell Line, TumorEndothelial CellsE-SelectinFlow CytometryFluorescent Antibody TechniqueHumansLewis X AntigenLung NeoplasmsMicroscopy, ConfocalNeoplasm InvasivenessTumor Necrosis Factor-alphaE-SelectinLewis X AntigenSELE protein, humanTumor Necrosis Factor-alphaadhesionbrain metastasisCD15CD62E

Identifiers

PMID26472821
PMCPMC4827040
OpenAlexW2169826577

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.