Evidence map›Paper›PMID 26472619›Full record

ArticleDevelopmental and comparative immunology2016

Structural and functional characterisation of ferret interleukin-2.

Bin Ren, William J McKinstry, Tam Pham, Janet Newman, Daniel S Layton, Andrew G Bean, Zhenjun Chen, Karen L Laurie, Kathryn Borg, Ian G Barr and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental and comparative immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Bin RenCSIRO Manufacturing, Parkville, VIC 3052, Australia.
William J McKinstryCSIRO Manufacturing, Parkville, VIC 3052, Australia.
Tam PhamCSIRO Manufacturing, Parkville, VIC 3052, Australia.
Janet NewmanCSIRO Manufacturing, Parkville, VIC 3052, Australia.
Daniel S LaytonCSIRO Health and Biosecurity, Geelong, VIC 3219, Australia.
Andrew G BeanCSIRO Health and Biosecurity, Geelong, VIC 3219, Australia.
Zhenjun ChenDepartment of Microbiology and Immunology, The University of Melbourne at the Doherty Institute, Melbourne, VIC 3000, Australia.
Karen L LaurieWHO Collaborating Centre for Reference and Research on Influenza (VIDRL), Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.
Kathryn BorgWHO Collaborating Centre for Reference and Research on Influenza (VIDRL), Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.
Ian G BarrWHO Collaborating Centre for Reference and Research on Influenza (VIDRL), Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.
Timothy E AdamsCSIRO Manufacturing, Parkville, VIC 3052, Australia. Electronic address: Tim.Adams@csiro.au.
CSIRO Manufacturing · AUCSIRO Health and Biosecurity · AUPeter Doherty Institute · AUUniversity of Melbourne · AUVictorian Infectious Diseases Reference Laboratory · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While the ferret is a valuable animal model for a number of human viral infections, such as influenza, Hendra and Nipah, evaluating the cellular immune response following infection has been hampered by the lack of a number of species-specific immunological reagents. Interleukin 2 (IL-2) is one such key cytokine. Ferret recombinant IL-2 incorporating a C-terminal histidine tag was expressed and purified and the three-dimensional structure solved and refined at 1.89 Å by X-ray crystallography, which represents the highest resolution and first non-human IL-2 structure. While ferret IL-2 displays the classic cytokine fold of the four-helix bundle structure, conformational flexibility was observed at the second helix and its neighbouring region in the bundle, which may result in the disruption of the spatial arrangement of residues involved in receptor binding interactions, implicating subtle differences between ferret and human IL-2 when initiating biological functions. Ferret recombinant IL-2 stimulated the proliferation of ferret lymph node cells and induced the expression of mRNA for IFN-γ and Granzyme A.

Indexed as

AnimalsCell ProliferationCells, CulturedCrystallography, X-RayFerretsGranzymesHumansInterferon-gammaInterleukin-2Lymph NodesProtein ConformationRecombinant ProteinsSpecies SpecificityStructural Homology, ProteinVirus DiseasesGranzymesInterferon-gammaInterleukin-2Recombinant ProteinsFerretLymphocyte proliferationmRNA inductionRecombinant interleukin-2X-ray structure

Identifiers

PMID26472619
PMCPMC7102629
OpenAlexW2178221216

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.