Evidence map›Paper›PMID 26472102›Full record

ArticleReproductive toxicology (Elmsford, N.Y.)2015

Comparison of toxicogenomic responses to phthalate ester exposure in an organotypic testis co-culture model and responses observed in vivo.

Sean Harris, Sanne A B Hermsen, Xiaozhong Yu, Sung Woo Hong, Elaine M Faustman

Open access · greenAbstract readComparative Study

In one paragraph

Article in Reproductive toxicology (Elmsford, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it

0cells of the map it votes in

8citing papers in PubMed

1.0field-weighted citation impact, top 24% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 19 citations in OpenAlex.

In Vitro and In Vivo Models for Drug Transport Across the Blood-Testis Barrier.Drug metabolism and disposition: the biological fate of chemicals · 2023
Review
The effects of gene × environment interactions on silver nanoparticle toxicity in the respiratory system: An adverse outcome pathway.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2021
Review
FutureTox IV Workshop Summary: Predictive Toxicology for Healthy Children.Toxicological sciences : an official journal of the Society of Toxicology · 2021
Review
Use of the Adverse Outcome Pathway (AOP) framework to evaluate species concordance and human relevance of Dibutyl phthalate (DBP)-induced male reproductive toxicity.Reproductive toxicology (Elmsford, N.Y.) · 2020
Review
In-vitro spermatogenesis through testis modelling: Toward the generation of testicular organoids.Andrology · 2020
Review
From the Cover: An Animal-Free In Vitro Three-Dimensional Testicular Cell Coculture Model for Evaluating Male Reproductive Toxicants.Toxicological sciences : an official journal of the Society of Toxicology · 2017
Article
High-Content Analysis Provides Mechanistic Insights into the Testicular Toxicity of Bisphenol A and Selected Analogues in Mouse Spermatogonial Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2017
Article
The presence of macrophages and inflammatory responses in an in vitro testicular co-culture model of male reproductive development enhance relevance to in vivo conditions.Toxicology in vitro : an international journal published in association with BIBRA · 2016
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sean HarrisDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98195, USA; Institute for Risk Analysis and Risk Communication, University of Washington, Seattle, WA 98105, USA.

Sanne A B HermsenDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98195, USA; Institute for Risk Analysis and Risk Communication, University of Washington, Seattle, WA 98105, USA.

Xiaozhong YuDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98195, USA; Institute for Risk Analysis and Risk Communication, University of Washington, Seattle, WA 98105, USA.

Sung Woo HongDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98195, USA; Institute for Risk Analysis and Risk Communication, University of Washington, Seattle, WA 98105, USA.

Elaine M FaustmanDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98195, USA; Institute for Risk Analysis and Risk Communication, University of Washington, Seattle, WA 98105, USA; Center for Ecogenetics and Environmental Health and Institute for Risk Analysis and Risk Communication, University of Washington, Seattle, WA 98105, USA. Electronic address: faustman@u.washington.edu.

University of Washington · US

Funding

XENOBIOTIC BIOTRANSFORMATION AND DISPOSITIONP30ES007033 · NIEHS · UNIVERSITY OF WASHINGTON · PI ALI SHOJAIE · 1995 to 2026

$42.5M

STUDY OF CELLULAR &MOLECULAR ASPECTS OF PESTICIDE NEURODEVELOPMENTAL TOXICITYP01ES009601 · NIEHS · UNIVERSITY OF WASHINGTON · PI FAUSTMAN, ELAINE M · 1999 to 2013

$8.7M

Intellectual and Developmental Disabilities Research CenterU54HD083091 · NICHD · UNIVERSITY OF WASHINGTON · PI GARDEN, GWENN A · 2014 to 2019

$7.9M

3D Testicular Cells Co-Culture Model for Reproductive and Developmental ToxicityU01FD004242 · FDA · UNIVERSITY OF WASHINGTON · PI FAUSTMAN, ELAINE M · 2011 to 2012

$757k

FDA HHS 1U01FD004242FDA HHS U01 FD004242NICHD NIH HHS 1 U54HD083091-01NICHD NIH HHS U54 HD083091NIEHS NIH HHS 5 P30 ES007033NIEHS NIH HHS P01 ES009601NIEHS NIH HHS P30 ES007033

6 · The paper itself

Abstract

We have developed a three-dimensional testicular co-culture system (3D-TCS) which mimics in vivo testes. In this study, transcriptomic responses to phthalate esters (PE's) were compared in the 3D-TCS with responses in rat testes in vivo. Microarray data from the 3D-TCS and from in vivo testes were used to compare changes in gene expression patterns after exposure to developmentally toxic (DTPE) or developmentally non-toxic (DNTPE) phthalate esters. DTPE treatments clustered separately from DNTPE treatments based on principle components analysis both in vitro and in vivo. Pathway analysis using GO-Elite software showed that terms relating to steroid metabolism or reproductive development were enriched both in vitro and in vivo after DTPE exposure. Processes such as cell cycle, cell proliferation and apoptosis were enriched for DTPE treatments in vitro, but not in vivo. Based on these analyses we concluded that transcriptomic responses in the 3D-TCS reflect key aspects of in vivo phthalate toxicity.

Indexed as

AnimalsAnimals, NewbornCells, CulturedCluster AnalysisCoculture TechniquesEstersGene Expression ProfilingGene Expression Regulation, DevelopmentalGestational AgeMaleOligonucleotide Array Sequence AnalysisPhthalic AcidsPrincipal Component AnalysisRats, Sprague-DawleySoftwareTestisEstersPhthalic AcidsIn vitroPhthalate estersReproductive toxicityTestesToxicogenomicTranscriptomic

Identifiers

PMID26472102

DOI10.1016/j.reprotox.2015.10.002

PMCPMC5166712

OpenAlexW1799928298

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.