Evidence map›Paper›PMID 26472042›Full record

ArticleMolecular cancer2015

Alcohol-dysregulated miR-30a and miR-934 in head and neck squamous cell carcinoma.

Maarouf A Saad, Selena Z Kuo, Elham Rahimy, Angela E Zou, Avinaash Korrapati, Mehran Rahimy, Elizabeth Kim, Hao Zheng, Michael Andrew Yu, Jessica Wang-Rodriguez and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cancer, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 108 citations in OpenAlex.

  1. Pooled it
  2. Review
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  7. Review
  8. The repertoire of mutational signatures in tobacco- and non-tobacco-induced oral cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023
    Review
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  18. Article
  19. Epimutational effects of electronic cigarettes.Environmental science and pollution research international · 2021
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Maarouf A SaadDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. masaad@ucsd.edu.
Selena Z KuoDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. szkuo@ucsd.edu.
Elham RahimyDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. elham.rahimy@yale.edu.
Angela E ZouDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. azou@ucsd.edu.
Avinaash KorrapatiDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. akorrapa@ucsd.edu.
Mehran RahimyDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. mrahimy@ucsd.edu.
Elizabeth KimDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. elkim@ucsd.edu.
Hao ZhengDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. h3zheng@ucsd.edu.
Michael Andrew YuDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. michael.yu@my.rfums.org.
Jessica Wang-RodriguezDepartment of Pathology, Veterans Administration Health Care System, San Diego, CA, USA. jessica.wang-rodriguez@va.gov.
Weg M OngkekoDivision of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. wongkeko@ucsd.edu.
University of California, San Diego · USUnited States Department of Veterans Affairs · US

Funding

Non-coding RNAs in salivary gland tumorR21DE023242 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ONGKEKO, RUTHERFORD M · 2012 to 2013
$417k
NIDCR NIH HHS DE023242NIDCR NIH HHS R21 DE023242
6 · The paper itself

Abstract

backgroundAlcohol consumption is a well-established risk factor for head and neck squamous cell carcinoma (HNSCC); however, the molecular mechanisms by which alcohol promotes HNSCC pathogenesis and progression remain poorly understood. Our study sought to identify microRNAs that are dysregulated in alcohol-associated HNSCC and investigate their contribution to the malignant phenotype.

methodUsing RNA-sequencing data from 136 HNSCC patients, we compared the expression levels of 1,046 microRNAs between drinking and non-drinking cohorts. Dysregulated microRNAs were verified by qRT-PCR in normal oral keratinocytes treated with biologically relevant doses of ethanol and acetaldehyde. The most promising microRNA candidates were investigated for their effects on cellular proliferation and invasion, sensitivity to cisplatin, and expression of cancer stem cell genes. Finally, putative target genes were identified and evaluated in vitro to further establish roles for these miRNAs in alcohol-associated HNSCC.

resultsFrom RNA-sequencing analysis we identified 8 miRNAs to be significantly upregulated in alcohol-associated HNSCCs. qRT-PCR experiments determined that among these candidates, miR-30a and miR-934 were the most highly upregulated in vitro by alcohol and acetaldehyde. Overexpression of miR-30a and miR-934 in normal and HNSCC cell lines produced up to a 2-fold increase in cellular proliferation, as well as induction of the anti-apoptotic gene BCL-2. Upon inhibition of these miRNAs, HNSCC cell lines exhibited increased sensitivity to cisplatin and reduced matrigel invasion. miRNA knockdown also indicated direct targeting of several tumor suppressor genes by miR-30a and miR-934.

conclusionsAlcohol induces the dysregulation of miR-30a and miR-934, which may play crucial roles in HNSCC pathogenesis and progression. Future investigation of the alcohol-mediated pathways effecting these transformations will prove valuable for furthering the understanding and treatment of alcohol-associated HNSCC.

Indexed as

AcetaldehydeAdultAlcoholsApoptosisCarcinoma, Squamous CellCell Line, TumorCell ProliferationEthanolFemaleGene Expression Regulation, NeoplasticHead and Neck NeoplasmsHumansMaleMicroRNAsMiddle AgedReverse Transcriptase Polymerase Chain ReactionAcetaldehydeAlcoholsEthanolMicroRNAsMIRN30b microRNA, human

Identifiers

PMID26472042
PMCPMC4608114
OpenAlexW1950892484

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.