ArticleScientific reports2015
Using AIE Luminogen for Long-term and Low-background Three-Photon Microscopic Functional Bioimaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Aggregation-induced emission luminescence for angiography and atherosclerotic diagnosis.iScience · 2024Review
- Phototoxic effects of nonlinear optical microscopy on cell cycle, oxidative states, and gene expression.Scientific reports · 2022Article
- Activation of apoptosis by rationally constructing NIR amphiphilic AIEgens: surmounting the shackle of mitochondrial membrane potential for amplified tumor ablation.Chemical science · 2021Article
- Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications.Frontiers in chemistry · 2020Review
- Enhanced three-photon activity triggered by the AIE behaviour of a novel terpyridine-based Zn(ii) complex bearing a thiophene bridge.Chemical science · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent probes are one of the most popularly used bioimaging markers to monitor metabolic processes of living cells. However, long-term light excitation always leads to photobleaching of fluorescent probes, unavoidable autofluorescence as well as photodamage of cells. To overcome these limitations, we synthesized a type of photostable luminogen named TPE-TPP with an aggregation induced emission (AIE) characteristic, and achieved its three-photon imaging with femtosecond laser excitation of 1020 nm. By using TPE-TPP as fluorescent probes, three-photon microscopy under 1020 nm excitation showed little photo-damage, as well as low autofluorescence to HeLa cells. Due to the AIE effect, the TPE-TPP nanoaggregates uptaken by cells were resistant to photobleaching under three-photon excitation for an extended period of time. Furthermore, we demonstrated that for the present TPE-TPP AIE the three-photon microscopy (with 1020 nm excitation) had a better signal to noise ratio than the two-photon microscopy (with 810 nm excitation) in tissue imaging.
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