ArticlePloS one2015
Single-Cell Expression Profiling Reveals a Dynamic State of Cardiac Precursor Cells in the Early Mouse Embryo.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- SpatialDG: a novel spatial domain identification method for spatially resolved transcriptomics data based on dual-graph neural network.Briefings in bioinformatics · 2026Article
- CITED Proteins in Cardiac Development and Lifelong Heart Function.Journal of personalized medicine · 2025Review
- Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells.Stem cell research & therapy · 2025Article
- Article
- A Mesp1-dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors.Development (Cambridge, England) · 2023Article
- Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart.NPJ Regenerative medicine · 2023Article
- Concomitant genetic defects potentiate the adverse impact of prenatal alcohol exposure on cardiac outflow tract maturation.Birth defects research · 2022Article
- Control of cardiomyocyte differentiation timing by intercellular signaling pathways.Seminars in cell & developmental biology · 2021Review
- Review
- Cardiopharyngeal Progenitor Specification: Multiple Roads to the Heart and Head Muscles.Cold Spring Harbor perspectives in biology · 2020Review
- Transcriptional Profiling of Single Cardiomyocytes in Health and Disease.Current cardiology reports · 2020Review
- Integrative Cluster Analysis of Whole Hearts Reveals Proliferative Cardiomyocytes in Adult Mice.Cells · 2020Article
- Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice.Scientific reports · 2019Article
- Development and evolution of the metazoan heart.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles.Cell stem cell · 2019Article
- Precardiac organoids form two heart fields via Bmp/Wnt signaling.Nature communications · 2018Article
- Single Cell Gene Expression to Understand the Dynamic Architecture of the Heart.Frontiers in cardiovascular medicine · 2018Review
- Earlier and broader roles of Mesp1 in cardiovascular development.Cellular and molecular life sciences : CMLS · 2017Review
- Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle.Nature communications · 2017Article
- GFRA2 Identifies Cardiac Progenitors and Mediates Cardiomyocyte Differentiation in a RET-Independent Signaling Pathway.Cell reports · 2016Article
Corrections and comments
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Authors and funding
17 authors at 3 institutions in 3 countries.
Funding
Abstract
In the early vertebrate embryo, cardiac progenitor/precursor cells (CPs) give rise to cardiac structures. Better understanding their biological character is critical to understand the heart development and to apply CPs for the clinical arena. However, our knowledge remains incomplete. With the use of single-cell expression profiling, we have now revealed rapid and dynamic changes in gene expression profiles of the embryonic CPs during the early phase after their segregation from the cardiac mesoderm. Progressively, the nascent mesodermal gene Mesp1 terminated, and Nkx2-5+/Tbx5+ population rapidly replaced the Tbx5low+ population as the expression of the cardiac genes Tbx5 and Nkx2-5 increased. At the Early Headfold stage, Tbx5-expressing CPs gradually showed a unique molecular signature with signs of cardiomyocyte differentiation. Lineage-tracing revealed a developmentally distinct characteristic of this population. They underwent progressive differentiation only towards the cardiomyocyte lineage corresponding to the first heart field rather than being maintained as a progenitor pool. More importantly, Tbx5 likely plays an important role in a transcriptional network to regulate the distinct character of the FHF via a positive feedback loop to activate the robust expression of Tbx5 in CPs. These data expands our knowledge on the behavior of CPs during the early phase of cardiac development, subsequently providing a platform for further study.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.