ArticleJournal of the American Chemical Society2015
Discovery of Unclustered Fungal Indole Diterpene Biosynthetic Pathways through Combinatorial Pathway Reassembly in Engineered Yeast.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Discovery and Biosynthesis of Sesquiterpene Polyketide Ester from an Atypical Terpene and Highly Reducing Polyketide Pathway.JACS Au · 2026Article
- Revolutionizing sweetness: the multifaceted health benefits of fermented stevia.Applied microbiology and biotechnology · 2026Review
- Complete biosynthesis of phenylspirodrimanes with unclustered genes inActa pharmaceutica Sinica. B · 2026Article
- Biosynthesis of sydonol reveals a new bisabolene cyclase and an unusual P450 aromatase.RSC chemical biology · 2025Article
- Genome Mining of Isoindolinone-Containing Peptide Natural Products.Journal of the American Chemical Society · 2025Article
- Context matters: assessing the impacts of genomic background and ecology on microbial biosynthetic gene cluster evolution.mSystems · 2025Review
- Total biosynthesis of cotylenin diterpene glycosides as 14-3-3 protein-protein interaction stabilizers.Chemical science · 2025Article
- Fragment-Guided Genome Mining of Octacyclic Cyclophane Alkaloids from Fungi.Journal of the American Chemical Society · 2024Article
- β-Terrecyclene synthase constructs the quadrane backbone in terrecyclic acid biosynthesis.Chemical science · 2024Article
- Discovery and Characterization of Pyridoxal 5'-Phosphate-Dependent Cycloleucine Synthases.Journal of the American Chemical Society · 2024Article
- Class II terpene cyclases: structures, mechanisms, and engineering.Natural product reports · 2024Review
- Indole Diterpene Derivatives from theMolecules (Basel, Switzerland) · 2023Article
- Functional Crosstalk between Discrete Indole Terpenoid Gene Clusters inOrganic letters · 2023Article
- A heterologous expression platform inChemical science · 2023Article
- Fungal P450 Deconstructs the 2,5-Diazabicyclo[2.2.2]octane RingJournal of the American Chemical Society · 2023Article
- Biosynthesis of AS2077715 and Funiculosin: Pathway Reconstitution and Identification of Enzymes that Form the All-Journal of the American Chemical Society · 2023Article
- Genome mining of cryptic bisabolenes that were biosynthesized by intramembrane terpene synthases fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Characterization and catalytic investigation of fungal single-module nonribosomal peptide synthetase in terpene-amino acid meroterpenoid biosynthesis.Journal of industrial microbiology & biotechnology · 2023Article
- Generation of Alternate Indole Diterpene Architectures in Two Species ofJournal of the American Chemical Society · 2023Article
- Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining.Natural product reports · 2023Review
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Authors and funding
10 authors.
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Abstract
The structural diversity and biological activities of fungal indole diterpenes (IDTs) are generated in large part by the IDT cyclases (IDTCs). Identifying different IDTCs from IDT biosynthetic pathways is therefore important toward understanding how these enzymes introduce chemical diversity from a common linear precursor. However, IDTCs involved in the cyclization of the well-known aflavinine subgroup of IDTs have not been discovered. Here, using Saccharomyces cerevisiae as a heterologous host and a phylogenetically guided enzyme mining approach, we combinatorially assembled IDT biosynthetic pathways using IDTCs homologues identified from different fungal hosts. We identified the genetically standalone IDTCs involved in the cyclization of aflavinine and anominine and produced new IDTs not previously isolated. The cyclization mechanisms of the new IDTCs were proposed based on the yeast reconstitution results. Our studies demonstrate heterologous pathway assembly is a useful tool in the reconstitution of unclustered biosynthetic pathways.
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