ArticleThe Journal of biological chemistry2015
AztD, a Periplasmic Zinc Metallochaperone to an ATP-binding Cassette (ABC) Transporter System in Paracoccus denitrificans.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Zinc-metallochaperones ofMicrobiology spectrum · 2025Article
- Structure and Function of the Zinc Binding Protein ZrgA fromInternational journal of molecular sciences · 2022Article
- Conformational flexibility in the zinc solute-binding protein ZnuA.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Contributions of Conformational Flexibility to High-Affinity Zinc Binding in the Solute Binding Protein AztC.ACS omega · 2022Article
- Structural Features Mediating Zinc Binding and Transfer in the AztABCD Zinc Transporter System.Biomolecules · 2020Article
- Oligopeptide-binding protein from nontypeableThe Journal of biological chemistry · 2019Article
- Two ABC Transporters and a Periplasmic Metallochaperone Participate in Zinc Acquisition in Paracoccus denitrificans.Biochemistry · 2019Article
- Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins.Communications biology · 2019Article
- The crystal structure of the Yersinia pestis iron chaperone YiuA reveals a basic triad binding motif for the chelated metal.Acta crystallographica. Section D, Structural biology · 2017Article
- Mechanisms of zinc binding to the solute-binding protein AztC and transfer from the metallochaperone AztD.The Journal of biological chemistry · 2017Article
- Zinc-Dependent Transcriptional Regulation inFrontiers in microbiology · 2017Article
- The biological inorganic chemistry of zinc ions.Archives of biochemistry and biophysics · 2016Article
- Bacterial Strategies to Maintain Zinc Metallostasis at the Host-Pathogen Interface.The Journal of biological chemistry · 2016Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial ATP-binding cassette (ABC) transporters of transition metals are essential for acquisition of necessary elements from the environment. A large number of Gram-negative bacteria, including human pathogens, have a fourth conserved gene of unknown function adjacent to the canonical permease, ATPase, and solute-binding protein (SBP) genes of the AztABC zinc transporter system. To assess the function of this putative accessory factor (AztD) from Paracoccus denitrificans, we have analyzed its transcriptional regulation, metal binding properties, and interaction with the SBP (AztC). Transcription of the aztD gene is significantly up-regulated under conditions of zinc starvation. Recombinantly expressed AztD purifies with slightly substoichiometric zinc from the periplasm of Escherichia coli and is capable of binding up to three zinc ions with high affinity. Size exclusion chromatography and a simple intrinsic fluorescence assay were used to determine that AztD as isolated is able to transfer bound zinc nearly quantitatively to apo-AztC. Transfer occurs through a direct, associative mechanism that prevents loss of metal to the solvent. These results indicate that AztD is a zinc chaperone to AztC and likely functions to maintain zinc homeostasis through interaction with the AztABC system. This work extends our understanding of periplasmic zinc trafficking and the function of chaperones in this process.
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