ArticleMicrobial biotechnology2016
The development of genetic and molecular markers to register and commercialize Penicillium rubens (formerly Penicillium oxalicum) strain 212 as a biocontrol agent.
Article in Microbial biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Environmental Compatibility ofJournal of fungi (Basel, Switzerland) · 2025Article
- Hybrid assembly ofIMA fungus · 2025Article
- An efficient Agrobacterium-mediated system based on the pyrG auxotrophic marker for recombinant expression in the filamentous fungus Penicillium rubens.Biotechnology letters · 2023Article
- Isolation and Molecular Characterization of IndigenousMicroorganisms · 2023Article
- Identification and characterization of biocontrol agent Lysinibacillus boronitolerans P42 against Cerrena unicolor that causes root rot of arecanut palm.Archives of microbiology · 2023Article
- Isolation and Identification ofArchives of Razi Institute · 2022Article
- Comparative analysis ofFrontiers in microbiology · 2022Article
- Using High-Throughput Amplicon Sequencing to Evaluate Intragenomic Variation and Accuracy in Species Identification ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Exploring the Extracellular Macromolecular Composition of Crude Extracts ofJournal of fungi (Basel, Switzerland) · 2020Article
- Transformation ofFrontiers in microbiology · 2018Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Penicillium oxalicum strain 212 (PO212) is an effective biocontrol agent (BCA) against a large number of economically important fungal plant pathogens. For successful registration as a BCA in Europe, PO212 must be accurately identified. In this report, we describe the use of classical genetic and molecular markers to characterize and identify PO212 in order to understand its ecological role in the environment or host. We successfully generated pyrimidine (pyr-) auxotrophic mutants. In addition we also designed specific oligonucleotides for the pyrF gene at their untranslated regions for rapid and reliable identification and classification of strains of P. oxalicum and P. rubens, formerly P. chrysogenum. Using these DNA-based technologies, we found that PO212 is a strain of P. rubens, and is not a strain of P. oxalicum. This work presents PO212 as the unique P. rubens strain to be described as a BCA and the information contained here serves for its registration and commercialization in Europe.
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Registered trials
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