ArticleBMC complementary and alternative medicine2015
Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells.
Article in BMC complementary and alternative medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 156 citations in OpenAlex.
- The Effect of Iranian Propolis on Glucose Metabolism, Lipid Profile, Insulin Resistance, Renal Function and Inflammatory Biomarkers in Patients with Type 2 Diabetes Mellitus: A Randomized Double-Blind Clinical Trial.Scientific reports · 2019Trial
- Toxic Metal Content in Mexican Propolis: A Human Health Risk Assessment.Foods (Basel, Switzerland) · 2026Article
- Adjustment of Wettability in Biomaterials: Evaluation of the Impact of Red Propolis Fractions on Electrospun Poly(ε-caprolactone).Chemistry & biodiversity · 2026Article
- Ultrasound-Assisted Pressurized Fluid Extraction of Antioxidant and Anticancer Molecules from a Mangaba, Cambuí and Red Propolis Blend.Molecules (Basel, Switzerland) · 2025Article
- Effect of Brazilian red propolis on microtensile bond strength and sealing ability of the methacrylate-based bonding agents.Journal of clinical and experimental dentistry · 2025Article
- Propolis Stands out as a Multifaceted Natural Product: Meta-Analysis on Its Sources, Bioactivities, Applications, and Future Perspectives.Life (Basel, Switzerland) · 2025Review
- Effect of Propolis on PPP2R1A and Apoptosis in Cancer Cells.Biochemistry research international · 2025Article
- Apoptosis, Cell Cycle and Differentiation Effects of Propolis Extracted in Olive Oil on HL-60 AML Cells: A Flow Cytometric Analysis.Analytical cellular pathology (Amsterdam) · 2025Article
- Development of a Benzophenone-Free Red Propolis Extract and Evaluation of Its Efficacy against Colon Carcinogenesis.Pharmaceuticals (Basel, Switzerland) · 2024Article
- P-coumaric Acid: Advances in Pharmacological Research Based on Oxidative Stress.Current topics in medicinal chemistry · 2024Review
- Anti-Molecules (Basel, Switzerland) · 2023Article
- Brazilian Amazon Red Propolis: Leishmanicidal Activity and Chemical Composition of a New Variety of Red Propolis.Metabolites · 2023Article
- Propolis-Based Nanostructured Lipid Carriers for α-Mangostin Delivery: Formulation, Characterization, and In Vitro Antioxidant Activity Evaluation.Molecules (Basel, Switzerland) · 2023Article
- Combination therapy: synergism among three plant extracts against selected pathogens.BMC research notes · 2023Article
- Review
- Propolis: A Detailed Insight of Its Anticancer Molecular Mechanisms.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells.Antioxidants (Basel, Switzerland) · 2023Article
- Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis.Antioxidants (Basel, Switzerland) · 2022Article
- Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP).Molecules (Basel, Switzerland) · 2022Article
- Red propolis exhibits chemopreventive effect associated with antiproliferative and anti-inflammatory activities.Toxicology research · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe implementation of new public healthcare models that stimulate the use of natural products from traditional medicine, as a so-called integrated medicine, refers to an approach that use best of both conventional medicine and traditional medicine. Propolis is a widely used natural product by different ancient cultures and known to exhibit biological activities beneficial for health. The large number of studies conducted with propolis had shown that its chemical composition differs as a function of the climate, plant diversity and bee species and plays an important role on its therapeutic properties. The aim of this study was to analyse the phytochemical profile of the ethanolic extract of red propolis (EEP) and its fractionation, antioxidant action of EEP and its fractions hexane, cloroform and ethyl acetate and cytotoxic activity of EEP on human tumour cell lines SF-295 (glioblastoma), OVCAR-8 (ovary) and HCT-116 (colon).
methodsEEP was obtained by maceration with absolute ethanol, then it was concentrated in rotaevaporator up to complete evaporation of the solvent. The crude extract was fractionated with hexane, ethyl acetate, chloroform and methanol and they were subjected to phytochemical screening and total phenolic compounds. Antioxidant activity of EEP and fractions was done by means of the 2,2-diphenyl-1-picryhydrazyl (DPPH) method. Biomarkers of red propolis were identified by LC-Orbitrap-FTMS. To assess cytotoxic activity of the extract, cells were exposed to EEP over 72 h. Cell viability was assessed by means of MTT assay. The percentage of cell growth inhibition (IC50) was analysed by means of non-linear regression, and the absorbance values of the various investigated concentrations were subjected to one-factor analysis of variance (ANOVA) followed by Tukey's or Tamhane's tests (α = 0.05).
resultsThe results obtained using phytochemical screening and LC-Orbitrap-FTMS indicated the presence of phlobaphene tannins, catechins, chalcones, aurones, flavonones, flavonols, xanthones, pentacyclic triterpenoids and guttiferones in Brazilian red propolis. EEP and its hexane, chloroform and ethyl acetate fractions obtained by liquid-liquid partitioning exhibited satisfactory antioxidant percentages. EEP (IC50 < 34.27 μg/mL) exhibited high levels of cytotoxicity on all human tumour cell lines tested when compared to negative control.
conclusionsC-Orbitrap-FTMS was useful to establish the chemical profile of the red propolis. Brazilian red propolis has antioxidant properties and decreases substantially the percentage of cell survival of human tumour cells; thus, it has potential to serve as an anticancer drug.
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