ArticleMolecular oral microbiology2016
High-throughput sequencing reveals key genes and immune homeostatic pathways activated in myeloid dendritic cells by Porphyromonas gingivalis 381 and its fimbrial mutants.
Article in Molecular oral microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
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- Dendritic cells a critical link to alveolar bone loss and systemic disease risk in periodontitis: Immunotherapeutic implications.Periodontology 2000 · 2022Review
- Do Oral Pathogens Inhabit the Eye and Play a Role in Ocular Diseases?Journal of clinical medicine · 2022Review
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- Exacerbation of AMD Phenotype in Lasered CNV Murine Model by Dysbiotic Oral Pathogens.Antioxidants (Basel, Switzerland) · 2021Article
- Microbial Lipid A Remodeling Controls Cross-Presentation Efficiency and CD8 T Cell Priming by Modulating Dendritic Cell Function.Infection and immunity · 2021Article
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- Invasion of Human Retinal Pigment Epithelial Cells by Porphyromonas gingivalis leading to Vacuolar/Cytosolic localization and Autophagy dysfunction In-Vitro.Scientific reports · 2020Article
- Oral Health and Cancer.Current oral health reports · 2019Article
- Immunization with cell-free-generated vaccine protects from Porphyromonas gingivalis-induced alveolar bone loss.Journal of clinical periodontology · 2019Article
- Mucosal Immunity and the FOXO1 Transcription Factors.Frontiers in immunology · 2019Review
- Disruption of Immune Homeostasis in Human Dendritic Cells via Regulation of Autophagy and Apoptosis byFrontiers in immunology · 2019Article
- Oral Pathobiont Activates Anti-Apoptotic Pathway, Promoting both Immune Suppression and Oncogenic Cell Proliferation.Scientific reports · 2018Article
- The function of dendritic cells in modulating the host response.Molecular oral microbiology · 2018Review
- Maturation of the Mfa1 Fimbriae in the Oral PathogenFrontiers in cellular and infection microbiology · 2018Article
- Genes Contributing toFrontiers in cellular and infection microbiology · 2017Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The human microbiome consists of highly diverse microbial communities that colonize our skin and mucosal surfaces, aiding in maintenance of immune homeostasis. The keystone pathogen Porphyromonas gingivalis induces a dysbiosis and disrupts immune homeostasis through as yet unclear mechanisms. The fimbrial adhesins of P. gingivalis facilitate biofilm formation, invasion of and dissemination by blood dendritic cells; hence, fimbriae may be key factors in disruption of immune homeostasis. In this study we employed RNA-sequencing transcriptome profiling to identify differentially expressed genes (DEGs) in human monocyte-derived dendritic cells (MoDCs) in response to in vitro infection/exposure by Pg381 or its isogenic mutant strains that solely express minor-Mfa1 fimbriae (DPG3), major-FimA fimbriae (MFI) or are deficient in both fimbriae (MFB) relative to uninfected control. Our results yielded a total of 479 DEGs that were at least two-fold upregulated and downregulated in MoDCs significantly (P ≤ 0.05) by all four strains and certain DEGs that were strain-specific. Interestingly, the gene ontology biological and functional analysis shows that the upregulated genes in DPG3-induced MoDCs were more significant than other strains and associated with inflammation, immune response, anti-apoptosis, cell proliferation, and other homeostatic functions. Both transcriptome and quantitative polymerase chain reaction results show that DPG3, which solely expresses Mfa1, increased ZNF366, CD209, LOX1, IDO1, IL-10, CCL2, SOCS3, STAT3 and FOXO1 gene expression. In conclusion, we have identified key DC-mediated immune homeostatic pathways that could contribute to dysbiosis in periodontal infection with P. gingivalis.
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