ArticleBMC genetics2015
MicroRNA profiles reveal female allotetraploid hybrid fertility.
Article in BMC genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- Hybridization and gene expression: Beyond differentially expressed genes.Molecular ecology · 2025Review
- Comparative Transcriptome Analysis Revealed Genes Involved in Sexual and Polyploid Growth Dimorphisms in Loach (Biology · 2021Article
- Molecular Mechanisms Regulating Muscle Plasticity in Fish.Animals : an open access journal from MDPI · 2020Review
- CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates.Frontiers in genetics · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe bisexual fertile tetraploid fish is important in biological evolution. Tetraploid fish fertility is the key factor for stable inheritance. Therefore, elucidating tetraploid fish fertility at the molecular level is essential. MicroRNAs regulate gene expression and are involved in many aspects of gonad development.
methodsTotal RNA was isolated using TRIzol, followed by constructing small RNA libraries. And then, the qualified libraries were sequenced with the HiSeq 2500 SE50 system. The obtained clean reads were analyzed to identify conserved and novel miRNAs, and evaluate the expression, and also predict the target genes. The differential expressions of miRNAs were confirmed by RT-PCR.
resultsIn this study, allotetraploid hybrid fish (4nAT) and diploid red crucian carp (RCC) ovaries were used to compare miRNA profiles. The results indicated that most of the highly expressed miRNAs were closely correlated with ovary maturation, and displayed no significant differences in expression. Moreover, 34 up-regulated and nine down-regulated miRNAs were found in 4nAT. The differentially expressed miRNAs were primarily involved in metabolism, defense mechanisms, and cytoskeleton production.
conclusionsThis is the first study to provide new epigenetic evidences for tetraploid fish fertility and phenotypic changes as a result of increased ploidy.
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Registered trials
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