ArticlePloS one2015
Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Direct Production of 2-Butanol from Glucose by RecombinantInternational journal of molecular sciences · 2026Article
- Analysis and application of volatile metabolic profiles ofRSC advances · 2024Article
- Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria: Current state of the art.Journal of pharmaceutical analysis · 2024Review
- Urinary VOCs as biomarkers of early stage lung tumour development in mice.Cancer biomarkers : section A of Disease markers · 2024Article
- Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion.Microorganisms · 2023Review
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- GC-MS profiling of volatile metabolites produced byFrontiers in molecular biosciences · 2022Article
- Feasible-metabolic-pathway-exploration technique using chemical latent space.Bioinformatics (Oxford, England) · 2020Article
- Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.Biotechnology for biofuels · 2020Article
- Urinary volatile metabolites of amygdala-kindled mice reveal novel biomarkers associated with temporal lobe epilepsy.Scientific reports · 2019Article
- Production of C2-C4 diols from renewable bioresources: new metabolic pathways and metabolic engineering strategies.Biotechnology for biofuels · 2017Review
- Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol.Biotechnology for biofuels · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2-Butanone is an important commodity chemical of wide application in different areas. In this study, Klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. By introducing the diol dehydratase from Lactobacillus brevis and deleting the ldhA gene encoding lactate dehydrogenase, the engineered K. pneumoniae was able to accumulate 246 mg/L of 2-butanone in shake flask. With further optimization of culture condition, the titer of 2-butanone was increased to 450 mg/L. This study lays the basis for developing an efficient biological process for 2-butanone production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.