ArticleAngewandte Chemie (International ed. in English)2015
Ribosome Subunit Stapling for Orthogonal Translation in E. coli.
Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 77 citations in OpenAlex.
- Role of Ribosomal Protein bS1 in Orthogonal mRNA Start Codon Selection.Biochemistry · 2025Article
- Assembly of functional microbial ecosystems: from molecular circuits to communities.FEMS microbiology reviews · 2024Review
- Reaching New Heights in Genetic Code Manipulation with High Throughput Screening.Chemical reviews · 2024Review
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Co-translational Installation of Posttranslational Modifications by Non-canonical Amino Acid Mutagenesis.Chembiochem : a European journal of chemical biology · 2023Article
- Automated 3D Design and Evaluation of RNA Nanostructures with RNAMake.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Computationally-guided design and selection of high performing ribosomal active site mutants.Nucleic acids research · 2022Article
- Mobile Translation Systems Generate Genomically EngineeredACS synthetic biology · 2022Article
- Improving the Efficiency and Orthogonality of Genetic Code Expansion.Biodesign research · 2022Review
- Specialised ribosomes as versatile regulators of gene expression.RNA biology · 2022Review
- Genetic Code Expansion: A Brief History and Perspective.Biochemistry · 2021Review
- Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation.Cell · 2021Article
- Orthogonal translation enables heterologous ribosome engineering in E. coli.Nature communications · 2021Article
- Synthetic Biological Circuits within an Orthogonal Central Dogma.Trends in biotechnology · 2021Review
- Mechanistic studies of non-canonical amino acid mutagenesis.Methods in enzymology · 2021Article
- Mutational characterization and mapping of the 70S ribosome active site.Nucleic acids research · 2020Article
- A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea.Nucleic acids research · 2020Article
- Strategies for in vitro engineering of the translation machinery.Nucleic acids research · 2020Review
- Single enzyme RT-PCR of full-length ribosomal RNA.Synthetic biology (Oxford, England) · 2020Article
- Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins.PloS one · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The creation of orthogonal large and small ribosomal subunits, which interact with each other but not with endogenous ribosomal subunits, would extend our capacity to create new functions in the ribosome by making the large subunit evolvable. To this end, we rationally designed a ribosomal RNA that covalently links the ribosome subunits via an RNA staple. The stapled ribosome is directed to an orthogonal mRNA, allowing the introduction of mutations into the large subunit that reduce orthogonal translation, but have minimal effects on cell growth. Our approach provides a promising route towards orthogonal subunit association, which may enable the evolution of key functional centers in the large subunit, including the peptidyl-transferase center, for unnatural polymer synthesis in cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.