ArticlePloS one2015
Overexpression of Three Glucosinolate Biosynthesis Genes in Brassica napus Identifies Enhanced Resistance to Sclerotinia sclerotiorum and Botrytis cinerea.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed.
- Application of Metabolomics in Defence Responses ofMetabolites · 2026Review
- Integrating host-pathogen interaction genomics to boost wheat defense against septoria tritici blotch.BMC genomics · 2025Article
- Integrated Transcriptome and Metabolome Analysis Reveals the Resistance Mechanisms ofInternational journal of molecular sciences · 2025Article
- Genome-wide association studies identify loci controlling specialized seed metabolites in Arabidopsis.Plant physiology · 2024Article
- Spectral Reflectance Indexes Reveal Differences in the Physiological Status ofPlants (Basel, Switzerland) · 2023Article
- Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways.Plant communications · 2023Review
- Genome-Wide Association Study of Glucosinolate Metabolites (mGWAS) inPlants (Basel, Switzerland) · 2023Article
- Genetic improvement of tocotrienol content enhances the oxidative stability of canola oil.Frontiers in plant science · 2023Article
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- The Characterization of theInternational journal of molecular sciences · 2022Article
- Glucosinolate biosynthesis: role of MAM synthase and its perspectives.Bioscience reports · 2021Article
- Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach.Microbiology spectrum · 2021Article
- Improvement of glucosinolates by metabolic engineering inaBIOTECH · 2021Review
- Overexpression ofFrontiers in plant science · 2021Article
- Transcriptome Analysis Reveals the Complex Molecular Mechanisms ofFrontiers in plant science · 2021Article
- Development of TransgenicPlants (Basel, Switzerland) · 2020Review
- Understanding Host-Pathogen Interactions inPlants (Basel, Switzerland) · 2020Review
- Dissection of genetic architecture for glucosinolate accumulations in leaves and seeds of Brassica napus by genome-wide association study.Plant biotechnology journal · 2020Article
- Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.).Scientific reports · 2020Article
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7 authors.
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Abstract
Sclerotinia sclerotiorum and Botrytis cinerea are notorious plant pathogenic fungi with an extensive host range including Brassica crops. Glucosinolates (GSLs) are an important group of secondary metabolites characteristic of the Brassicales order, whose degradation products are proving to be increasingly important in plant protection. Enhancing the defense effect of GSL and their associated degradation products is an attractive strategy to strengthen the resistance of plants by transgenic approaches. We generated the lines of Brassica napus with three biosynthesis genes involved in GSL metabolic pathway (BnMAM1, BnCYP83A1 and BnUGT74B1), respectively. We then measured the foliar GSLs of each transgenic lines and inoculated them with S. sclerotiorum and B. cinerea. Compared with the wild type control, over-expressing BnUGT74B1 in B. napus increased the aliphatic and indolic GSL levels by 1.7 and 1.5 folds in leaves respectively; while over-expressing BnMAM1 or BnCYP83A1 resulted in an approximate 1.5-fold higher only in the aliphatic GSL level in leaves. The results of plant inoculation demonstrated that BnUGT74B1-overexpressing lines showed less severe disease symptoms and tissue damage compared with the wild type control, but BnMAM1 or BnCYP83A1-overexpressing lines showed no significant difference in comparison to the controls. These results suggest that the resistance to S. sclerotiorum and B. cinerea in B. napus could be enhanced through tailoring the GSL profiles by transgenic approaches or molecular breeding, which provides useful information to assist plant breeders to design improved breeding strategies.
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