Evidence map›Paper›PMID 26464991›Full record

ArticleeNeuro

The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells

Colette Moses, Lachlan P G Wheeler, Chrisna J LeVaillant, Anne Kramer, Marisa Ryan, Greg S Cozens, Anil Sharma, Margaret A Pollett, Jennifer Rodger, Alan R Harvey

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
9.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 103 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 2 countries.

Colette MosesSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Lachlan P G WheelerSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Chrisna J LeVaillantSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Anne KramerSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Marisa RyanSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Greg S CozensSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Anil SharmaSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia ; Department of Neuroscience, Karolinska Institutet , 17177 Stockholm, Sweden.
Margaret A PollettSchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia.
Jennifer RodgerSchool of Animal Biology, The University of Western Australia , Perth, Western Australia 6009, Australia ; Western Australian Neuroscience Research Institute , Nedlands, Western Australia 6009, Australia.
Alan R HarveySchool of Anatomy, Physiology, and Human Biology, The University of Western Australia , Perth, Western Australia 6009, Australia ; Western Australian Neuroscience Research Institute , Nedlands, Western Australia 6009, Australia.
The University of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Similar to neurons in the peripheral nervous system, immature CNS-derived RGCs become dependent on target-derived neurotrophic support as their axons reach termination sites in the brain. To study the factors that influence this developmental transition we took advantage of the fact that rat RGCs are born, and target innervation occurs, over a protracted period of time. Early-born RGCs have axons in the SC by birth (P0), whereas axons from late-born RGCs do not innervate the SC until P4-P5. Birth dating RGCs using EdU allowed us to identify RGCs (1) with axons still growing toward targets, (2) transitioning to target dependence, and (3) entirely dependent on target-derived support. Using laser-capture microdissection we isolated ∼34,000 EdU(+) RGCs and analyzed transcript expression by custom qPCR array. Statistical analyses revealed a difference in gene expression profiles in actively growing RGCs compared with target-dependent RGCs, as well as in transitional versus target-dependent RGCs. Prior to innervation RGCs expressed high levels of BDNF and CNTFR α but lower levels of neurexin 1 mRNA. Analysis also revealed greater expression of transcripts for signaling molecules such as MAPK, Akt, CREB, and STAT. In a supporting in vitro study, purified birth-dated P1 RGCs were cultured for 24-48 h with or without BDNF; lack of BDNF resulted in significant loss of early-born but not late-born RGCs. In summary, we identified several important changes in RGC signaling that may form the basis for the switch from target independence to dependence.

Indexed as

BDNFneurotrophic factorsprogrammed cell deathretinal ganglion cellstarget dependence

Identifiers

PMID26464991
PMCPMC4586937
OpenAlexW2161340614

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.