ArticleNucleic acids research2015
SKI2 mediates degradation of RISC 5'-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 96 citations in OpenAlex.
- Functional allelic diversity of PTGS components revealed by a high-throughput RUBY-based screening.The Plant journal : for cell and molecular biology · 2026Article
- An active RNA transport mechanism into plant vacuoles.Nucleic acids research · 2026Article
- RNA Tailing by Nucleotidyltransferases in Plants: Mechanisms, Functions, and Biological Significance.Plants (Basel, Switzerland) · 2026Review
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Response to "The action of Arabidopsis DICER-LIKE2 in plant growth inhibition".The Plant cell · 2025Article
- Sporophyte-directed gametogenesis in Arabidopsis.Nature plants · 2025Article
- RNA interference and turnover in plants -a complex partnership.Frontiers in plant science · 2025Review
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- Multi-transcriptomics identifies targets of the endoribonuclease DNE1 and highlights its coordination with decapping.The Plant cell · 2024Article
- Functions and mechanisms of RNA tailing by nucleotidyl transferase proteins in plants.Frontiers in plant science · 2024Review
- Quantitative Investigation ofInternational journal of molecular sciences · 2023Article
- Genome-wide identification of DCL, AGO, and RDR gene families in wheat (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Crosstalk between RNA silencing and RNA quality control in plants.BMB reports · 2023Review
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- Functions and mechanisms of RNA helicases in plants.Journal of experimental botany · 2023Review
- Tweaking the Small Non-Coding RNAs to Improve Desirable Traits in Plant.International journal of molecular sciences · 2023Review
- microRNA production inFrontiers in plant science · 2023Review
- Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Catalytic activities, molecular connections, and biological functions of plant RNA exosome complexes.The Plant cell · 2022Review
- Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in Arabidopsis.Nucleic acids research · 2022Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small regulatory RNAs are fundamental in eukaryotic and prokaryotic gene regulation. In plants, an important element of post-transcriptional control is effected by 20-24 nt microRNAs (miRNAs) and short interfering RNAs (siRNAs) bound to the ARGONAUTE1 (AGO1) protein in an RNA induced silencing complex (RISC). AGO1 may cleave target mRNAs with small RNA complementarity, but the fate of the resulting cleavage fragments remains incompletely understood. Here, we show that SKI2, SKI3 and SKI8, subunits of a cytoplasmic cofactor of the RNA exosome, are required for degradation of RISC 5', but not 3'-cleavage fragments in Arabidopsis. In the absence of SKI2 activity, many miRNA targets produce siRNAs via the RNA-dependent RNA polymerase 6 (RDR6) pathway. These siRNAs are low-abundant, and map close to the cleavage site. In most cases, siRNAs were produced 5' to the cleavage site, but several examples of 3'-spreading were also identified. These observations suggest that siRNAs do not simply derive from RDR6 action on stable 5'-cleavage fragments and hence that SKI2 has a direct role in limiting secondary siRNA production in addition to its function in mediating degradation of 5'-cleavage fragments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.