ArticleThe Journal of cell biology1989
Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Functional improvement of mutant keratin cells on addition of desmin: an alternative approach to gene therapy for dominant diseases.Gene therapy · 2004Article
- Demyelination and axonal dystrophy in alpha A-crystallin transgenic mice.International journal of experimental pathology · 2000Article
- Mechanical alterations in smooth muscle from mice lacking desmin.Journal of muscle research and cell motility · 1998Article
- Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation.The American journal of pathology · 1997Article
- Peripheral neuropathy in mice transgenic for a human MDR3 P-glycoprotein mini-gene.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Human multidrug resistance 3-P-glycoprotein expression in transgenic mice induces lens membrane alterations leading to cataract.The Journal of cell biology · 1996Article
- Changes in expression of differentiation markers between normal ovarian cells and derived tumors.The American journal of pathology · 1993Article
- Desmin/vimentin intermediate filaments are dispensable for many aspects of myogenesis.The Journal of cell biology · 1991Article
- Assembly of amino-terminally deleted desmin in vimentin-free cells.The Journal of cell biology · 1990Article
- A single human keratin 18 gene is expressed in diverse epithelial cells of transgenic mice.The Journal of cell biology · 1990Article
- Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia.The American journal of pathology · 1990Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The coding region of the hamster desmin gene was fused to the 5' flanking sequences of the hamster vimentin gene and introduced into the germ line of mice. The expression of this intermediate filament gene construct (pVDes) was analyzed at the RNA and protein level in transgenic mice as well as in fibroblast cell lines and primary hepatocyte cultures derived from these mice. In all transgenic mice, the pVDes-encoded protein was coexpressed with mouse vimentin in a tissue-specific fashion and was indistinguishable from normal hamster desmin. Culturing of transgenic hepatocytes induced desmin expression indicating that 3.2 kbp of the vimentin gene 5' region regulates both tissue-specific and tissue culture-induced intermediate filament protein expression. Immunohistochemical staining and double-label immunoelectron microscopy of cultured transgenic fibroblasts showed that the pVDes protein assembled into intermediate filaments which colocalized with the mouse vimentin filaments. Endogenous vimentin RNA levels were not influenced by high-level pVDes expression. The coexpression of desmin and vimentin in nonmuscle cells did not result in detectable developmental, morphological, or physiological abnormalities.
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Registered trials
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