ArticleBMC genomics2015
Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 21 citations in OpenAlex.
- Functional characterization of transcriptional enhancers in an Anopheles genetic locus controlling natural resistance to the malaria parasite, Plasmodium falciparum.Epigenetics & chromatin · 2025Article
- Article
- The Anopheles leucine-rich repeat protein APL1C is a pathogen binding factor recognizing Plasmodium ookinetes and sporozoites.PLoS pathogens · 2024Article
- Evolution and assembly ofOpen biology · 2023Article
- Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti.Nature communications · 2022Article
- Genetics and immunity of Anopheles response to the entomopathogenic fungus Metarhizium anisopliae overlap with immunity to Plasmodium.Scientific reports · 2022Article
- Susceptibility of Field-CollectedGenes · 2021Article
- Comprehensive Ecological and Geographic Characterization of Eukaryotic and Prokaryotic Microbiomes in AfricanFrontiers in microbiology · 2021Article
- Exposure of Anopheles mosquitoes to trypanosomes reduces reproductive fitness and enhances susceptibility to Plasmodium.PLoS neglected tropical diseases · 2020Article
- BSA-Seq Discovery and Functional Analysis of Candidate Hessian Fly (Frontiers in plant science · 2020Article
- Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii.Scientific reports · 2019Article
- Transmission traits of malaria parasites within the mosquito: Genetic variation, phenotypic plasticity, and consequences for control.Evolutionary applications · 2018Article
- Human Genomic Loci Important in Common Infectious Diseases: Role of High-Throughput Sequencing and Genome-Wide Association Studies.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2018Review
- Identifying coevolving loci using interspecific genetic correlations.Ecology and evolution · 2017Article
- Rapidly Evolving Toll-3/4 Genes Encode Male-Specific Toll-Like Receptors in Drosophila.Molecular biology and evolution · 2017Article
- Recent advances in malaria genomics and epigenomics.Genome medicine · 2016Review
- Plant-Mediated Effects on Mosquito Capacity to Transmit Human Malaria.PLoS pathogens · 2016Article
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Authors and funding
17 authors at 7 institutions in 5 countries.
Funding
Abstract
backgroundThe genome-wide association study (GWAS) techniques that have been used for genetic mapping in other organisms have not been successfully applied to mosquitoes, which have genetic characteristics of high nucleotide diversity, low linkage disequilibrium, and complex population stratification that render population-based GWAS essentially unfeasible at realistic sample size and marker density.
methodsWe designed a novel mapping strategy for the mosquito system that combines the power of linkage mapping with the resolution afforded by genetic association. We established founder colonies from West Africa, controlled for diversity, linkage disequilibrium and population stratification. Colonies were challenged by feeding on the infectious stage of the human malaria parasite, Plasmodium falciparum, mosquitoes were phenotyped for parasite load, and DNA pools for phenotypically similar mosquitoes were Illumina sequenced. Phenotype-genotype mapping was carried out in two stages, coarse and fine.
resultsIn the first mapping stage, pooled sequences were analysed genome-wide for intervals displaying relativereduction in diversity between phenotype pools, and candidate genomic loci were identified for influence upon parasite infection levels. In the second mapping stage, focused genotyping of SNPs from the first mapping stage was carried out in unpooled individual mosquitoes and replicates. The second stage confirmed significant SNPs in a locus encoding two Toll-family proteins. RNAi-mediated gene silencing and infection challenge revealed that TOLL 11 protects mosquitoes against P. falciparum infection.
conclusionsWe present an efficient and cost-effective method for genetic mapping using natural variation segregating in defined recent Anopheles founder colonies, and demonstrate its applicability for mapping in a complex non-model genome. This approach is a practical and preferred alternative to population-based GWAS for first-pass mapping of phenotypes in Anopheles. This design should facilitate mapping of other traits involved in physiology, epidemiology, and behaviour.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.