ArticleScientific reports2015
Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 126 citations in OpenAlex.
- Composite Liquid Marble Templated Millimetric Capsule With Tunable Rigidity, Porosity, and Thermal Reconfigurability Toward 3D Cell Culture.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Liquid Marbles and Drops on Superhydrophobic Surfaces: Interfacial Aspects and Dynamics of Formation: A Review.ACS omega · 2024Review
- Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates.Animals : an open access journal from MDPI · 2023Article
- Spheroid Engineering in Microfluidic Devices.ACS omega · 2023Review
- Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.PloS one · 2023Article
- Liquid marbles, floating droplets: preparations, properties, operations and applications.RSC advances · 2022Review
- Microtubular Assessment of C6 Rat Glioma Cell Spheroids Developed in Transparent Liquid Marbles or Hanging Drops.Biology · 2022Article
- The promoting effects of activated olfactory ensheathing cells on angiogenesis after spinal cord injury through the PI3K/Akt pathway.Cell & bioscience · 2022Article
- Flipped Well-Plate Hanging-Drop Technique for Growing Three-Dimensional Tumors.Frontiers in bioengineering and biotechnology · 2022Article
- From cell spheroids to vascularized cancer organoids: Microfluidic tumor-on-a-chip models for preclinical drug evaluations.Biomicrofluidics · 2021Review
- Analyzing Olfactory Neuron Precursors Non-Invasively Isolated through NADH FLIM as a Potential Tool to Study Oxidative Stress in Alzheimer's Disease.International journal of molecular sciences · 2021Review
- Current Advances in 3D Tissue and Organ Reconstruction.International journal of molecular sciences · 2021Review
- Role of cellulose family in fibril organization of collagen for forming 3D cancer spheroids:BioImpacts : BI · 2021Article
- Micro Elastofluidics: Elasticity and Flexibility for Efficient Microscale Liquid Handling.Micromachines · 2020Article
- All roads lead to Rome: the many ways to pluripotency.Journal of assisted reproduction and genetics · 2020Review
- Article
- Durable superamphiphobic silica aerogel surfaces for the culture of 3D cellular spheroids.National science review · 2019Article
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids.Methods in molecular biology (Clifton, N.J.) · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other cells in three dimensions. Transplantation of OECs is being trialled for repair of the paralysed spinal cord, with promising but variable results and thus the therapy needs improving. To date, studies of OEC behaviour in a multicellular environment have been hampered by the lack of suitable three-dimensional cell culture models. Here, we exploit the floating liquid marble, a liquid droplet coated with hydrophobic powder and placed on a liquid bath. The presence of the liquid bath increases the humidity and minimises the effect of evaporation. Floating liquid marbles allow the OECs to freely associate and interact to produce OEC spheroids with uniform shapes and sizes. In contrast, a sessile liquid marble on a solid surface suffers from evaporation and the cells aggregate with irregular shapes. We used floating liquid marbles to co-culture OECs with Schwann cells and astrocytes which formed natural structures without the confines of gels or bounding layers. This protocol can be used to determine how OECs and other cell types associate and interact while forming complex cell structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.