Evidence map›Paper›PMID 26462274›Full record

ArticleBMJ open gastroenterology2015

Reduced keratin expression in colorectal neoplasia and associated fields is reversible by diet and resection.

Caroline A Evans, Ria Rosser, Jennifer S Waby, Josselin Noirel, Daphne Lai, Phillip C Wright, Elizabeth A Williams, Stuart A Riley, Jonathan P Bury, Bernard M Corfe

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMJ open gastroenterology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Caroline A EvansDepartment of Chemical and Biological Engineering , ChELSI Institute, University of Sheffield , Sheffield , UK.
Ria RosserMolecular Gastroenterology Research Group, Department of Oncology , University of Sheffield, The Medical School , Sheffield , UK.
Jennifer S WabyMolecular Gastroenterology Research Group, Department of Oncology , University of Sheffield, The Medical School , Sheffield , UK ; Department of Biological Sciences , The University of Hull , Hull , UK.
Josselin NoirelDepartment of Chemical and Biological Engineering , ChELSI Institute, University of Sheffield , Sheffield , UK ; Conservatoire National des Arts et Mmétiers , Paris , France.
Daphne LaiMolecular Gastroenterology Research Group, Department of Oncology , University of Sheffield, The Medical School , Sheffield , UK ; Department of Geography , University of Sheffield , Sheffield , UK.
Phillip C WrightDepartment of Chemical and Biological Engineering , ChELSI Institute, University of Sheffield , Sheffield , UK.
Elizabeth A WilliamsHuman Nutrition Unit, Department of Oncology , University of Sheffield, The Medical School , Sheffield , UK.
Stuart A RileyDepartment of Gastroenterology , Northern General Hospital , Sheffield , UK.
Jonathan P BuryDepartment of Pathology , Royal Hallamshire Hospital , Sheffield , UK.
Bernard M CorfeMolecular Gastroenterology Research Group, Department of Oncology , University of Sheffield, The Medical School , Sheffield , UK ; Insigneo Institute for in Silico Medicine, The University of Sheffield , Sheffield , UK.
University of Sheffield · GBConservatoire National des Arts et Métiers · FRInsigneo · GBNorthern General Hospital · GBRoyal Hallamshire Hospital · GBUniversity of Hull · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPatients with adenomatous colonic polyps are at increased risk of developing further polyps suggesting field-wide alterations in cancer predisposition. The current study aimed to identify molecular alterations in the normal mucosa in the proximity of adenomatous polyps and to assess the modulating effect of butyrate, a chemopreventive compound produced by fermentation of dietary residues.

methodsA cross-sectional study was undertaken in patients with adenomatous polyps: biopsy samples were taken from the adenoma, and from macroscopically normal mucosa on the contralateral wall to the adenoma and from the mid-sigmoid colon. In normal subjects biopsies were taken from the mid-sigmoid colon. Biopsies were frozen for proteomic analysis or formalin-fixed for immunohistochemistry. Proteomic analysis was undertaken using iTRAQ workflows followed by bioinformatics analyses. A second dietary fibre intervention study arm used the same endpoints and sampling strategy at the beginning and end of a high-fibre intervention.

resultsKey findings were that keratins 8, 18 and 19 were reduced in expression level with progressive proximity to the lesion. Lesional tissue exhibited multiple K8 immunoreactive bands and overall reduced levels of keratin. Biopsies from normal subjects with low faecal butyrate also showed depressed keratin expression. Resection of the lesion and elevation of dietary fibre intake both appeared to restore keratin expression level.

conclusionChanges in keratin expression associate with progression towards neoplasia, but remain modifiable risk factors. Dietary strategies may improve secondary chemoprevention. TRIAL REGISTRATION NUMBER: ISRCTN90852168.

Indexed as

ADENOMABUTYRATECYTOKERATINSDIETARY FIBRE

Identifiers

PMID26462274
PMCPMC4599164
OpenAlexW2158652500

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.