ArticlePloS one2015
Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Engineering a high-sugar tolerant strain of Saccharomyces cerevisiae for efficient trehalose production using a cell surface display approach.Bioresources and bioprocessing · 2024Article
- Characterization of cold-active trehalose synthase from Pseudarthrobacter sp. for trehalose bioproduction.Bioresources and bioprocessing · 2023Article
- Integrated Whole-Cell Biocatalysis for Trehalose Production from Maltose Using PermeabilizedJournal of microbiology and biotechnology · 2022Article
- Efficient Biosynthesis of 10-Hydroxy-2-decenoic Acid Using a NAD(P)H Regeneration P450 System and Whole-Cell Catalytic Biosynthesis.ACS omega · 2022Article
- Evaluation of trehalase as an enhancer for a green biocide in the mitigation of Desulfovibrio vulgaris biocorrosion of carbon steel.Bioprocess and biosystems engineering · 2022Article
- Stoichiometric Conversion of Maltose for Biomanufacturing byBiodesign research · 2022Article
- An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes.World journal of microbiology & biotechnology · 2021Review
- Recent Advances in Producing Sugar Alcohols and Functional Sugars by EngineeringFrontiers in bioengineering and biotechnology · 2021Review
- Improved Expression and Optimization of Trehalose Synthase by Regulation of PScientific reports · 2019Article
- Preservation of protein expression systems at elevated temperatures for portable therapeutic production.Journal of the Royal Society, Interface · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trehalose is a non-reducing disaccharide, which can protect proteins, lipid membranes, and cells from desiccation, refrigeration, dehydration, and other harsh environments. Trehalose can be produced by different pathways and trehalose synthase pathway is a convenient, practical, and low-cost pathway for the industrial production of trehalose. In this study, 3 candidate treS genes were screened from genomic databases of Pseudomonas and expressed in Escherichia coli. One of them from P. stutzeri A1501 exhibited the best transformation ability from maltose into trehalose and the least byproduct. Thus, whole cells of this recombinant E. coli were used as biocatalyst for trehalose production. In order to improve the conversion rate of maltose to trehalose, optimization of the permeabilization and biotransformation were carried out. Under optimal conditions, 92.2 g/l trehalose was produced with a high productivity of 23.1 g/(l h). No increase of glucose was detected during the whole course. The biocatalytic process developed in this study might serve as a candidate for the large scale production of trehalose.
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Registered trials
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