ArticlePloS one2015
Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 67 citations in OpenAlex.
- Three novel bacterial species isolated from plasma-treated soil: Hymenobacter plasmatis sp. nov., Flavitalea plasmicola sp. nov. and Mucilaginibacter tolerans sp. nov.Antonie van Leeuwenhoek · 2026Article
- Submerged Spark Plasma Discharges for the Removal of Antibiotics, Antibiotic-Resistant Bacteria, and Resistance Genes.Plasma chemistry and plasma processing · 2026Article
- Treatment of Clinically Important Bacteria With Cold Atmospheric Plasma.Microbial biotechnology · 2025Review
- Plasma Optimization as a Novel Tool to Explore Plant-Microbe Interactions in Climate Smart Agriculture.Microorganisms · 2025Review
- Article
- Understanding the transition to viable but non-culturable state in Escherichia coli W3110: a comprehensive analysis of potential spectrochemical biomarkers.World journal of microbiology & biotechnology · 2024Article
- Delivery Systems for Plasma-reactive Species and their Applications in the Field of Biomedicine.Current drug delivery · 2024Review
- Anti-inflammatory effects of cold atmospheric plasma irradiation on the THP-1 human acute monocytic leukemia cell line.PloS one · 2023Article
- YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response.iScience · 2022Article
- Oxidative lesions and post-treatment viability attenuation of listeria monocytogenes triggered by atmospheric non-thermal plasma.Journal of applied microbiology · 2022Article
- Toxicity Assessment of Long-Term Exposure to Non-Thermal Plasma Activated Water in Mice.International journal of molecular sciences · 2021Article
- Inactivation effects and mechanisms of plasma-activated water combined with sodium laureth sulfate (SLES) against Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2021Article
- Effect of Non-Thermal Atmospheric Plasma on Food-Borne Bacterial Pathogens on Ready-to Eat Foods: Morphological and Physico-Chemical Changes Occurring on the Cellular Envelopes.Foods (Basel, Switzerland) · 2020Article
- Use of a model to understand the synergies underlying the antibacterial mechanism of HScientific reports · 2020Article
- A Review on Non-thermal Atmospheric Plasma for Food Preservation: Mode of Action, Determinants of Effectiveness, and Applications.Frontiers in microbiology · 2019Review
- Cold Plasmas for Biofilm Control: Opportunities and Challenges.Trends in biotechnology · 2018Review
- Review of the methods for determination of reactive oxygen species and suggestion for their application in advanced oxidation induced by dielectric barrier discharges.Environmental science and pollution research international · 2018Article
- Article
- Involvement of multiple stressors induced by non-thermal plasma-charged aerosols during inactivation of airborne bacteria.PloS one · 2017Article
- Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently reported that phosphate-buffered saline (PBS) treated with nonthermal dielectric-barrier discharge plasma (plasma) acquires strong antimicrobial properties, but the mechanisms underlying bacterial inactivation were not known. The goal of this study is to understand the cellular responses of Escherichia coli and to investigate the properties of plasma-activated PBS. The plasma-activated PBS induces severe oxidative stress in E. coli cells and reactive-oxygen species scavengers, α-tocopherol and catalase, protect E. coli from cell death. Here we show that the response of E. coli to plasma-activated PBS is regulated by OxyR and SoxyRS regulons, and mediated predominantly through the expression of katG that deactivates plasma-generated oxidants. During compensation of E. coli in the absence of both katG and katE, sodA and sodB are significantly overexpressed in samples exposed to plasma-treated PBS. Microarray analysis found that up-regulation of genes involved in DNA repair, and E. coli expressing recA::lux fusion was extremely sensitive to the SOS response upon exposure to plasma-treated PBS. The cellular changes include rapid loss of E. coli membrane potential and membrane integrity, lipid peroxidation, accumulation of 8-hydroxy-deoxyguinosine (8OHdG), and severe oxidative DNA damage; reveal ultimate DNA disintegration, and cell death. Together, these data suggest that plasma-treated PBS contains hydrogen peroxide and superoxide like reactive species or/and their products which lead to oxidative changes to cell components, and are eventually responsible for cell death.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.