Evidence map›Paper›PMID 26460926›Full record

ArticleEuropean journal of immunology2016

Pattern recognition receptor mediated downregulation of microRNA-650 fine-tunes MxA expression in dendritic cells infected with influenza A virus.

Tica Pichulik, Elham Khatamzas, Xiao Liu, Oliver Brain, Magno Delmiro Garcia, Alasdair Leslie, Benedicte Danis, Alice Mayer, Dilair Baban, Jiannis Ragoussis and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 17 citations in OpenAlex.

  1. Functions and mechanisms of miR-650 in human diseases.Frontiers in molecular biosciences · 2026
    Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Role of microRNA and Oxidative Stress in Influenza A Virus Pathogenesis.International journal of molecular sciences · 2020
    Review
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  10. Article
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Tica PichulikMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Elham KhatamzasMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Xiao LiuDepartment of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany.
Oliver BrainMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Magno Delmiro GarciaDepartment of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany.
Alasdair LeslieMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Benedicte DanisMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Alice MayerMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Dilair BabanWellcome Trust Centre for Human Genetics, Oxford, UK.
Jiannis RagoussisWellcome Trust Centre for Human Genetics, Oxford, UK.
Alexander N R WeberDepartment of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany.
Alison SimmonsMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
John Radcliffe Hospital · GBUniversity of Tübingen · DECentre for Human Genetics · GBMRC Human Immunology Unit · GB

Funding

Department of Health NIHR-RP-R3-12-026Medical Research CouncilWellcome Trust 102974
6 · The paper itself

Abstract

MicroRNAs are important posttranscriptional regulators of gene expression, which have been shown to fine-tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR-650 as a novel PRR-responsive microRNA that is downregulated upon stimulation of primary human monocyte-derived dendritic cells (MDDCs) with a variety of different microbe-associated molecular patterns. A comprehensive target search combining in silico analysis, transcriptional profiling, and reporter assays reveals that miR-650 regulates several well-known interferon-stimulated genes, including IFIT2 and MXA. In particular, downregulation of miR-650 in influenza A infected MDDCs enhances the expression of MxA and may therefore contribute to the establishment of an antiviral state. Together these findings reveal a novel link between miR-650 and the innate immune response in human MDDCs.

Indexed as

Cells, CulturedDendritic CellsDown-RegulationFlow CytometryGene Expression RegulationHumansImmunity, InnateImmunoblottingInfluenza A virusMicroRNAsMicroscopy, ConfocalMyxovirus Resistance ProteinsOligonucleotide Array Sequence AnalysisReceptors, Pattern RecognitionSignal TransductionTransfectionMicroRNAsMIRN650 microRNA, humanMX1 protein, humanMyxovirus Resistance ProteinsReceptors, Pattern RecognitionDendritic cellsHost/pathogens interactionsInnate immunityISGsMicroRNA

Identifiers

PMID26460926
PMCPMC4738369
OpenAlexW2101517151

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.