Evidence map›Paper›PMID 26460850›Full record

ArticleSurgical infections2015

Monitoring Neutrophil-Expressed Cell Surface Esophageal Cancer Related Gene-4 after Severe Burn Injury.

Todd W Costantini, Raul Coimbra, Nicole E Lopez, Jeanne G Lee, Bruce Potenza, Alan Smith, Andrew Baird, Brian P Eliceiri

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Surgical infections, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Todd W CostantiniDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Raul CoimbraDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Nicole E LopezDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Jeanne G LeeDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Bruce PotenzaDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Alan SmithDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Andrew BairdDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.
Brian P EliceiriDivision of Trauma, Surgical Critical Care, and Burn, Department of Surgery, University of California San Diego Health Sciences , San Diego, California.

Funding

A Combinatorial Approach to Wound Healing for Protein, Gene and Cell TherapeuticsP20GM078421 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BAIRD, ANDREW · 2006 to 2009
$3.4M
Vascular mechanisms regulating breast cancer brain metastasisR01CA170140 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELICEIRI, BRIAN P, FELDING, BRUNIE H. · 2012 to 2016
$1.8M
NCI NIH HHS R01 CA170140NIGMS NIH HHS P20GM078421
6 · The paper itself

Abstract

backgroundWe identified recently esophageal cancer related gene-4 (ECRG4) as a candidate cytokine that is expressed on the surface of quiescent polymorphonuclear leukocytes (PMNs) and shed in response to ex vivo treatment with lipopolysaccharide. To investigate the potential biologic relevance of changes in cell surface ECRG4 in human samples, we performed a pilot study to examine a population of burn patients in whom blood could be analyzed prospectively. We hypothesized that cutaneous burn injury would alter cell surface expression of ECRG4 on PMNs.

methodsPatients admitted with more than 20% total burn surface area (TBSA) (n = 10) had blood collected at the time of admission and weekly thereafter. For comparison, blood was obtained from a control group of healthy human volunteers (n = 4). We used flow cytometry to measure changes in ECRG4(+) PMNs from patients during recovery from injury. Esophageal cancer related gene-4 expression at each time point was compared with the patient's clinical status based on a Multiple Organ Dysfunction (MOD) score.

resultsEsophageal cancer related gene-4 was detected on the PMN surface of cells collected from healthy volunteers, however, within 48 h of admission after burn injury (n = 10 patients), the number of PMNs with cell surface ECRG4 was decreased. Esophageal cancer related gene-4 expression in PMNs was re-established over the course of patient recovery, unless complications occurred. In this case, the decrease in cell surface ECRG4(+) PMNs preceded the clinical diagnosis of infectious complications and was reflected by increased organ injury scores.

conclusionFrom a small sample set, we were able to determine that PMN cell surface ECRG4 expression was decreased after burn injury and returned to baseline during recovery from injury. Although larger studies are needed to define the role of ECRG4 in human PMNs further, this report is the first assessment of cell surface ECRG4 protein in a patient population to support analogous findings in animal studies.

Indexed as

AdultAnimalsBurnsFlow CytometryHumansMaleMembrane ProteinsMiddle AgedNeoplasm ProteinsNeutrophilsPilot ProjectsProspective StudiesTumor Suppressor ProteinsYoung AdultECRG4 protein, humanMembrane ProteinsNeoplasm ProteinsTumor Suppressor Proteins

Identifiers

PMID26460850
PMCPMC4663648

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.