Evidence map›Paper›PMID 26460205›Full record

ArticleGastroenterology2016

Relationship Between Microbiota of the Colonic Mucosa vs Feces and Symptoms, Colonic Transit, and Methane Production in Female Patients With Chronic Constipation.

Gopanandan Parthasarathy, Jun Chen, Xianfeng Chen, Nicholas Chia, Helen M O'Connor, Patricia G Wolf, H Rex Gaskins, Adil E Bharucha

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gastroenterology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03279224 (A Randomized Controlled Trial of the Safety and Efficacy of Fecal Microbiota Transplantation in a Population With Bipolar Disorder), which is not on this map. Cited by 226 papers, 7 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
226citing papers in PubMed, 7 pooled it
12.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03279224 phase2 / phase3unknown statusnot on this mapstarted 2018, after this paper: background citation

A Randomized Controlled Trial of the Safety and Efficacy of Fecal Microbiota Transplantation in a Population With Bipolar Disorder

TypeinterventionalSponsorValerie TaylorRan2018 to 2022Enrolled35ConditionsBipolar DepressionArmsAllogenic FMT, Autologous FMT
3 · Its place in the literature

Who cites it

226 citing papers in PubMed, 7 syntheses or guidelines pooled it, 390 citations in OpenAlex.

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  7. GUT MICROBIOTA AND THE USE OF PROBIOTICS IN CONSTIPATION IN CHILDREN AND ADOLESCENTS: SYSTEMATIC REVIEW.Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao Paulo · 2020
    Pooled it
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166 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Gopanandan ParthasarathyDivision of Gastroenterology and Hepatology, Department of Medicine, Mayo Clinic, Rochester, Minnesota.
Jun ChenDivision of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota; Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota.
Xianfeng ChenDivision of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota.
Nicholas ChiaDivision of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota; Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota.
Helen M O'ConnorClinical Research and Trials Unit, Center for Clinical and Translational Science, Mayo Clinic, Rochester, Minnesota.
Patricia G WolfDepartment of Animal Sciences, Department of Pathobiology, Division of Nutritional Sciences, University of Illinois, Urbana-Champaign, Illinois.
H Rex GaskinsDepartment of Animal Sciences, Department of Pathobiology, Division of Nutritional Sciences, University of Illinois, Urbana-Champaign, Illinois.
Adil E BharuchaDivision of Gastroenterology and Hepatology, Department of Medicine, Mayo Clinic, Rochester, Minnesota. Electronic address: bharucha.adil@mayo.edu.
Mayo Clinic · USUniversity of Illinois Urbana-Champaign · USMayo Clinic in Florida · US

Funding

Mayo Clinic Center for Translational Science ActivitiesUL1TR000135 · NCATS · MAYO CLINIC ROCHESTER · PI KHOSLA, SUNDEEP · 2012 to 2015
$41.2M
PILOT AND FEASIBILTY PROGRAMP30DK084567 · NIDDK · MAYO CLINIC ROCHESTER · PI GREGORY J. GORES · 2009 to 2026
$22.2M
Pathophysiology of Anorectal DisordersR01DK078924 · NIDDK · MAYO CLINIC ROCHESTER · PI BHARUCHA, ADIL E. · 2008 to 2023
$5.0M
Microbial Metabolic Toxicity Drives Colon CancerR01CA179243 · NCI · MAYO CLINIC ROCHESTER · PI CHIA, NICHOLAS · 2014 to 2018
$2.0M
NCATS NIH HHS UL1 TR000135NCI NIH HHS R01 CA179243NIDDK NIH HHS P30 DK084567NIDDK NIH HHS R01 DK078924NIDDK NIH HHS R01 DK78924
6 · The paper itself

Abstract

BACKGROUND &

aimsIn fecal samples from patients with chronic constipation, the microbiota differs from that of healthy subjects. However, the profiles of fecal microbiota only partially replicate those of the mucosal microbiota. It is not clear whether these differences are caused by variations in diet or colonic transit, or are associated with methane production (measured by breath tests). We compared the colonic mucosal and fecal microbiota in patients with chronic constipation and in healthy subjects to investigate the relationships between microbiota and other parameters.

methodsSigmoid colonic mucosal and fecal microbiota samples were collected from 25 healthy women (controls) and 25 women with chronic constipation and evaluated by 16S ribosomal RNA gene sequencing (average, 49,186 reads/sample). We assessed associations between microbiota (overall composition and operational taxonomic units) and demographic variables, diet, constipation status, colonic transit, and methane production (measured in breath samples after oral lactulose intake).

resultsFourteen patients with chronic constipation had slow colonic transit. The profile of the colonic mucosal microbiota differed between constipated patients and controls (P < .05). The overall composition of the colonic mucosal microbiota was associated with constipation, independent of colonic transit (P < .05), and discriminated between patients with constipation and controls with 94% accuracy. Genera from Bacteroidetes were more abundant in the colonic mucosal microbiota of patients with constipation. The profile of the fecal microbiota was associated with colonic transit before adjusting for constipation, age, body mass index, and diet; genera from Firmicutes (Faecalibacterium, Lactococcus, and Roseburia) correlated with faster colonic transit. Methane production was associated with the composition of the fecal microbiota, but not with constipation or colonic transit.

conclusionsAfter adjusting for diet and colonic transit, the profile of the microbiota in the colonic mucosa could discriminate patients with constipation from healthy individuals. The profile of the fecal microbiota was associated with colonic transit and methane production (measured in breath), but not constipation.

Indexed as

Gastrointestinal MicrobiomeGastrointestinal TransitAdultAlgorithmsBacteriaBreath TestsCase-Control StudiesChronic DiseaseColonConstipationFecesFemaleHumansIntestinal MucosaMethaneMiddle AgedMethaneIrritable Bowel SyndromeMicrobiome

Identifiers

PMID26460205
PMCPMC4727996
OpenAlexW1915385926

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.