Evidence map›Paper›PMID 26460176›Full record

ArticleExperimental cell research2016

Hedgehog-driven myogenic tumors recapitulate skeletal muscle cellular heterogeneity.

Simone Hettmer, Michael M Lin, Daria Tchessalova, Sara J Tortorici, Alessandra Castiglioni, Tushar Desai, Junhao Mao, Andrew P McMahon, Amy J Wagers

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental cell research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Negative correlation of single-cellLife science alliance · 2021
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Simone HettmerDepartment of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA; Division of Pediatric Hematology and Oncology, Department of Pediatric and Adolescent Medicine, University Medical Center Freiburg, University of Freiburg, Germany. Electronic address: simone.hettmer@uniklinik-freiburg.de.
Michael M LinDepartment of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
Daria TchessalovaDepartment of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
Sara J TortoriciDepartment of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
Alessandra CastiglioniDepartment of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
Tushar DesaiDepartment of Medicine, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Junhao MaoDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Andrew P McMahonDepartment of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, CA 90089, USA.
Amy J WagersDepartment of Stem Cell and Regenerative Biology, Harvard University, Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
Harvard Stem Cell Institute · USHarvard University · USStanford University · USUniversity of Massachusetts Chan Medical School · USUniversity of Southern California · US

Funding

SPECIAL ASSAY COREP30DK036836 · NIDDK · JOSLIN DIABETES CENTER · PI JEAN E. SCHAFFER · 1986 to 2026
$50.5M
Regulation of polarity by Sonic hedgehog morphogen signalingR01NS033642 · NINDS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MCMAHON, ANDREW P. · 1995 to 2016
$5.7M
Regulation of CNS and Limb Polarity by Sonic HedgehogR37NS033642 · NINDS · HARVARD UNIVERSITY · PI MCMAHON, ANDREW P. · 2005 to 2011
$5.6M
NIDDK NIH HHS DK036836NIDDK NIH HHS P30 DK036836NINDS NIH HHS NS033642NINDS NIH HHS R01 NS033642NINDS NIH HHS R37 NS033642Wellcome Trust
6 · The paper itself

Abstract

Hedgehog (Hh) pathway activation in R26-SmoM2;CAGGS-CreER mice, which carry a tamoxifen-inducible activated Smoothened allele (SmoM2), results in numerous microscopic tumor foci in mouse skeletal muscle. These tumors exhibit a highly differentiated myogenic phenotype and resemble human fetal rhabdomyomas. This study sought to apply previously established strategies to isolate lineally distinct populations of normal mouse myofiber-associated cells in order to examine cellular heterogeneity in SmoM2 tumors. We demonstrate that established SmoM2 tumors are composed of cells expressing myogenic, adipocytic and hematopoietic lineage markers and differentiation capacity. SmoM2 tumors thus recapitulate the phenotypic and functional hetereogeneity observed in normal mouse skeletal muscle. SmoM2 tumors also contain an expanded population of PAX7+ and MyoD+ satellite-like cells with extremely low clonogenic activity. Selective activation of Hh signaling in freshly isolated muscle satellite cells enhanced terminal myogenic differentiation without stimulating proliferation. Our findings support the conclusion that SmoM2 tumors represent an aberrant skeletal muscle state and demonstrate that, similar to normal muscle, myogenic tumors contain functionally distinct cell subsets, including cells lacking myogenic differentiation potential.

Indexed as

AllelesAnimalsCell DifferentiationCell ProliferationFlow CytometryHedgehog ProteinsMiceMice, Inbred C57BLMuscle, SkeletalNeoplasms, Muscle TissuePhenotypeHedgehog ProteinsDifferentiationHedgehog signalingIntratumoral cellular heterogeneitySkeletal muscle

Identifiers

PMID26460176
PMCPMC4718790
OpenAlexW1953229888

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.