ArticleIranian biomedical journal2016
Modulation of Lipopolysaccharide Stimulated Nuclear Factor kappa B Mediated iNOS/NO Production by Bromelain in Rat Primary Microglial Cells.
Article in Iranian biomedical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Exploring the Therapeutic Potential of Bromelain: Applications, Benefits, and Mechanisms.Nutrients · 2024Pooled it
- The Effects of Bromelain Supplementation on Clinical Outcomes, Inflammatory and Oxidative Stress Biomarkers in Patients With Traumatic Brain Injury: A Randomized, Double-Blind Placebo-Controlled Clinical Trial.Food science & nutrition · 2026Article
- Potential Relevance of Amazonian Diet Components in Parkinson's Disease: An Integrative Review with Multivariate Analysis.Nutrients · 2026Review
- Bromelain in Obesity Therapy: A Review of Anti-Inflammatory and Metabolic Mechanisms.International journal of molecular sciences · 2025Review
- Effect of bromelain on ischemia-reperfusion injury in the torsion model created in polycystic and normal ovarian tissues.Frontiers in pharmacology · 2024Article
- Beneficial Properties of Bromelain.Nutrients · 2021Review
- The Impact of Antarctic Ice Microalgae Polysaccharides on D-Galactose-Induced Oxidative Damage in Mice.Frontiers in nutrition · 2021Article
- A Novel Biological Role of α-Mangostin via TAK1-NF-κB Pathway against Inflammatory.Inflammation · 2019Article
- PYNOD reduces microglial inflammation and consequent neurotoxicity upon lipopolysaccharides stimulation.Experimental and therapeutic medicine · 2018Article
- Implications of designing a bromelain loaded enteric nanoformulation on its stability and anti-inflammatory potential upon oral administration.RSC advances · 2018Article
- Phytanic Acid-Induced Neurotoxicological Manifestations and Apoptosis Ameliorated by Mitochondria-Mediated Actions of Melatonin.Molecular neurobiology · 2017Article
- Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through alteredAmerican journal of translational research · 2017Article
- Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice.Molecular medicine reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroglial cells act as the sentinel of the central nervous system .They are involved in neuroprotection but are highly implicated in neurodegeneration of the aging brain. When over-activated, microglia release pro-inflammatory factors, such as nitric oxide (NO) and cytokines, which are critical in eliciting neuroinflammatory responses associated with neurodegenerative diseases. This study examined whether bromelain, the pineapple-derived extract, may exert an anti-inflammatory effect in primary microglia and may be neuroprotective by regulating microglial activation.
methodsFollowing the isolation of neonatal rat primary microglial cells, the activation profile of microglia was investigated by studying the effects of bromelain (5, 10, 20, and 30 µg/ml) on the levels of NO, inducible nitric oxide synthase (iNOS), and nuclear factor kappa B (NF-κB) in microglia treated with lipopolysaccharide (LPS) (1 µg/ml). Data were analyzed using Student's t-test. P values less than 0.05 were considered to be statistically significant, compared with the LPS-treated group without bromelain.
resultsResults showed that pretreatment of rat primary microglia with bromelain, decreased the production of NO induced by LPS (1 µg/ml) treatment in a dose-dependent manner. Bromelain (30 µg/ml) also significantly reduced the expression of iNOS at mRNA level and NF-κB at protein level. Moreover, the study of mitochondrial activity in microglia indicated that bromelain had no cytotoxicity at any of the applied doses, suggesting that the anti-inflammatory effects of bromelain are not due to cell death.
conclusionBromelain can be of potential use as an agent for alleviation of symptoms in neurodegenerative diseases.
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