ArticleMalaria journal2015
Non-invasive surveillance for Plasmodium in reservoir macaque species.
Article in Malaria journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
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- Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil.Microorganisms · 2023Article
- The potential for zoonotic malaria transmission in five areas of Indonesia inhabited by non-human primates.Parasites & vectors · 2023Article
- Pathologic characteristics of infectious diseases in macaque monkeys used in biomedical and toxicologic studies.Journal of toxicologic pathology · 2023Review
- The history and current epidemiology of malaria in Kalimantan, Indonesia.Malaria journal · 2022Review
- Plasmodium pitheci malaria in Bornean orang-utans at a rehabilitation centre in West Kalimantan, Indonesia.Malaria journal · 2022Article
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- Primate malaria: An emerging challenge of zoonotic malaria in Indonesia.One health (Amsterdam, Netherlands) · 2022Review
- Zoonotic Blood-Borne Pathogens in Non-Human Primates in the Neotropical Region: A Systematic Review.Pathogens (Basel, Switzerland) · 2021Review
- Atlantic Forest Malaria: A Review of More than 20 Years of Epidemiological Investigation.Microorganisms · 2021Review
- Complexity of malaria transmission dynamics in the Brazilian Atlantic Forest.Current research in parasitology & vector-borne diseases · 2021Article
- Plasmodium knowlesi infecting humans in Southeast Asia: What's next?PLoS neglected tropical diseases · 2020Review
- Prevalence of Plasmodium parasites in non-human primates and mosquitoes in areas with different degrees of fragmentation in Colombia.Malaria journal · 2019Article
- Occurrence of Selected Zoonotic Fecal Pathogens and First Molecular Identification ofBioMed research international · 2019Article
- Wild bonobos host geographically restricted malaria parasites including a putative new Laverania species.Nature communications · 2017Article
- Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.Memorias do Instituto Oswaldo Cruz · 2016Article
- Primate malarias: Diversity, distribution and insights for zoonoticOne health (Amsterdam, Netherlands) · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrimates are important reservoirs for human diseases, but their infection status and disease dynamics are difficult to track in the wild. Within the last decade, a macaque malaria, Plasmodium knowlesi, has caused disease in hundreds of humans in Southeast Asia. In order to track cases and understand zoonotic risk, it is imperative to be able to quantify infection status in reservoir macaque species. In this study, protocols for the collection of non-invasive samples and isolation of malaria parasites from naturally infected macaques are optimized.
methodsPaired faecal and blood samples from 60 Macaca fascicularis and four Macaca nemestrina were collected. All animals came from Sumatra or Java and were housed in semi-captive breeding colonies around West Java. DNA was extracted from samples using a modified protocol. Nested polymerase chain reactions (PCR) were run to detect Plasmodium using primers targeting mitochondrial DNA. Sensitivity of screening faecal samples for Plasmodium was compared to other studies using Kruskal Wallis tests and logistic regression models.
resultsThe best primer set was 96.7 % (95 % confidence intervals (CI): 83.3-99.4 %) sensitive for detecting Plasmodium in faecal samples of naturally infected macaques (n = 30). This is the first study to produce definitive estimates of Plasmodium sensitivity and specificity in faecal samples from naturally infected hosts. The sensitivity was significantly higher than some other studies involving wild primates.
conclusionsFaecal samples can be used for detection of malaria infection in field surveys of macaques, even when there are no parasites visible in thin blood smears. Repeating samples from individuals will improve inferences of the epidemiology of malaria in wild primates.
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