ArticleScientific reports2015
A simple bridging flocculation assay for rapid, sensitive and stringent detection of gene specific DNA methylation.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 36 citations in OpenAlex.
- Reverse transcriptase recombinase polymerase amplification for detection of tomato spotted wilt orthotospovirus from crude plant extracts.Scientific reports · 2023Article
- Hybridization-based CpG methylation level detection using methyl-CpG-binding domain-fused luciferase.Analytical and bioanalytical chemistry · 2023Article
- Malaria diagnostic update: From conventional to advanced method.Journal of clinical laboratory analysis · 2022Review
- An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles.Biotechnology progress · 2018Article
- Recombinase polymerase amplification: Basics, applications and recent advances.Trends in analytical chemistry : TRAC · 2018Review
- Liquid-to-gel transition for visual and tactile detection of biological analytes.Chemical communications (Cambridge, England) · 2017Article
- Sensitive detection of miRNA by using hybridization chain reaction coupled with positively charged gold nanoparticles.Scientific reports · 2016Article
- A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.Scientific reports · 2016Article
- The influence of menstrual cycle and endometriosis on endometrial methylome.Clinical epigenetics · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples. We then coupled this with a DNA-mediated flocculation assay for rapid and low cost naked-eye binary evaluation of highly methylated genes in cell line and blood DNA. The low resource requirements of our method may enable widespread adoption of DNA methylation-based diagnostics in clinic and may be useful for small-scale research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.