ArticleFunctional & integrative genomics2016
RNA-Seq mediated root transcriptome analysis of Chlorophytum borivilianum for identification of genes involved in saponin biosynthesis.
Article in Functional & integrative genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 25 citations in OpenAlex.
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- Effective prediction of biosynthetic pathway genes involved in bioactive polyphyllins in Paris polyphylla.Communications biology · 2022Article
- Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome ofFrontiers in plant science · 2022Article
- Identification of novel microRNAs and their targets in Chlorophytum borivilianum by small RNA and degradome sequencing.Non-coding RNA research · 2019Article
- Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis inMolecules (Basel, Switzerland) · 2019Article
- Candidate genes of flavonoid biosynthesis in Selaginella bryopteris (L.) Baker identified by RNA-Seq.Functional & integrative genomics · 2018Article
- Review
- Transcriptomic profiling of developing fiber in levant cotton (Gossypium herbaceum L.).Functional & integrative genomics · 2018Article
- Small RNA profiling for identification of miRNAs involved in regulation of saponins biosynthesis in Chlorophytum borivilianum.BMC plant biology · 2017Article
- Transcriptome and metabolome analysis of Ferula gummosa Boiss. to reveal major biosynthetic pathways of galbanum compounds.Functional & integrative genomics · 2017Article
- Analysis of transcription factors among differentially expressed genes induced by drought stress in Populus davidiana.3 Biotech · 2017Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlorophytum borivilianum is an important species of liliaceae family, owing to its vital medicinal properties. Plant roots are used for aphrodisiac, adaptogen, anti-aging, health-restorative and health-promoting purposes. Saponins, are considered to be the principal bioactive components responsible for the wide variety of pharmacological properties of this plant. In the present study, we have performed de novo root transcriptome sequencing of C. borivilianum using Illumina Hiseq 2000 platform, to gain molecular insight into saponins biosynthesis. A total of 33,963,356 high-quality reads were obtained after quality filtration. Sequences were assembled using various programs which generated 97,344 transcripts with a size range of 100-5,216 bp and N50 value of 342. Data was analyzed against non-redundant proteins, gene ontology (GO), and enzyme commission (EC) databases. All the genes involved in saponins biosynthesis along with five full-length genes namely farnesyl pyrophosphate synthase, cycloartenol synthase, β-amyrin synthase, cytochrome p450, and sterol-3-glucosyltransferase were identified. Read per exon kilobase per million (RPKM)-based comparative expression profiling was done to study the differential regulation of the genes. In silico expression analysis of seven selected genes of saponin biosynthetic pathway was validated by qRT-PCR.
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