Evidence map›Paper›PMID 26457835›Full record

ArticleBiomaterials2016

Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells.

Allison I Hoch, Vaishali Mittal, Debika Mitra, Nina Vollmer, Christopher A Zikry, J Kent Leach

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 97 citations in OpenAlex.

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  7. [Advantages and prospects of cell derived decellularized extracellular matrix as tissue engineering scaffolds].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Allison I HochDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, United States.
Vaishali MittalDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, United States.
Debika MitraDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, United States.
Nina VollmerDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, United States.
Christopher A ZikryDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, United States.
J Kent LeachDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, United States; Department of Orthopaedic Surgery, School of Medicine, University of California, Davis, Sacramento, CA 95817, United States. Electronic address: jkleach@ucdavis.edu.
University of California, Davis · US

Funding

Training Program in Biomolecular TechnologyT32GM008799 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI LEACH, J. KENT · 2002 to 2016
$2.4M
Cell delivery for irradiated bone defectsR03DE021704 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2011 to 2012
$230k
NIDCR NIH HHS R03 DE021704NIDCR NIH HHS R03-DE021704NIGMS NIH HHS T32 GM008799NIGMS NIH HHS T32-GM008799
6 · The paper itself

Abstract

Prior to transplantation, mesenchymal stem/stromal cells (MSCs) can be induced toward the osteoblastic phenotype using a cocktail of soluble supplements. However, there is little evidence of differentiated MSCs directly participating in bone formation, suggesting that MSCs may either die or revert in phenotype upon transplantation. Cell-secreted decellularized extracellular matrices (DMs) are a promising platform to confer bioactivity and direct cell fate through the presentation of a complex and physiologically relevant milieu. Therefore, we examined the capacity of biomimetic DMs to preserve the mineral-producing phenotype upon withdrawal of the induction stimulus. Regardless of induction duration, ranging up to 6 weeks, MSCs exhibited up to a 5-fold reduction in osteogenic markers within 24 h following stimulus withdrawal. We show that seeding osteogenically induced MSCs on DMs yields up to 2-fold more calcium deposition than tissue culture plastic, and this improvement is at least partially mediated by increasing actin cytoskeletal tension via the ROCK II pathway. MSCs on DMs also secreted 25% more vascular endothelial growth factor (VEGF), a crucial endogenous proangiogenic factor that is abrogated during MSC osteogenic differentiation. The deployment of DMs into a subcutaneous ectopic site enhanced the persistence of MSCs 5-fold, vessel density 3-fold, and bone formation 2-fold more than MSCs delivered without DMs. These results underscore the need for deploying MSCs using biomaterial platforms such as DMs to preserve the in vitro-acquired mineral-producing phenotype and accelerate the process of bone repair.

Indexed as

Bone DevelopmentCell DifferentiationAnimalsCell ProliferationHumansMesenchymal Stem CellsMiceRheologyBoneDedifferentiationExtracellular matrixMesenchymal stem/stromal cellsOsteogenesis

Identifiers

PMID26457835
PMCPMC4661076
OpenAlexW2218281805

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.