Evidence map›Paper›PMID 26457519›Full record

ArticleActa crystallographica. Section F, Structural biology communications2015

Crystallization and preliminary X-ray diffraction studies of frutalin, an α-D-galactose-specific lectin from Artocarpus incisa seeds.

Ana Cristina de Oliveira Monteiro-Moreira, Humberto D'Muniz Pereira, Antonio Eufrasio Vieira Neto, Frederico Bruno Mendes Batista Moreno, Marina Duarte Pinto Lobo, Felipe Domingos de Sousa, Renato de Azevedo Moreira

Open access · bronzeAbstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Frutapin, a lectin fromBioscience reports · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ana Cristina de Oliveira Monteiro-MoreiraCenter of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
Humberto D'Muniz PereiraPhysics Institute of São Carlos, São Paulo University, Avenida Trabalhador São-carlense 400, Parque Arnold Schimidt, 13566-590 São Carlos-SP, Brazil.
Antonio Eufrasio Vieira NetoCenter of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
Frederico Bruno Mendes Batista MorenoDepartment of Biochemistry and Molecular Biology, Federal University of Ceará (UFC), Campus do Pici, Bloco 907, 60451-970 Fortaleza-CE, Brazil.
Marina Duarte Pinto LoboCenter of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
Felipe Domingos de SousaCenter of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
Renato de Azevedo MoreiraDepartment of Biochemistry and Molecular Biology, Federal University of Ceará (UFC), Campus do Pici, Bloco 907, 60451-970 Fortaleza-CE, Brazil.
Universidade de Fortaleza · BRUniversidade Federal do Ceará · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Frutalin is an α-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and is a powerful tool for tumour biomarker discovery. The crystallization and preliminary X-ray diffraction analysis of this lectin, which was isolated from Artocarpus incisa seeds, are reported here. Frutalin was purified and submitted to mass-spectrometric analysis. Diverse masses at approximately 16 kDa were observed in the deconvoluted spectra, which support the presence of isoforms. The best frutalin crystals were grown within a week in 0.1 M citric acid pH 3.5 which contained 25% PEG 3350 as a precipitant at 293 K, and diffracted to a maximum resolution of 1.81 Å. The monoclinic crystals belonged to space group I2, with unit-cell parameters a = 76.17, b = 74.56, c = 118.98 Å, β = 96.56°. A molecular-replacement solution was obtained which indicated the presence of four monomers per asymmetric unit. Crystallographic refinement of the structure is in progress.

Indexed as

ArtocarpusCrystallizationGalactoseGalectinsHydrogen-Ion ConcentrationLectinsMass SpectrometrySeedsX-Ray DiffractionfrutalinGalactoseGalectinsLectinsArtocarpus incisafrutalinjacalin lectin-related family

Identifiers

PMID26457519
PMCPMC4601592
OpenAlexW1954198936

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.