ArticleVirology2015
Replacement of the hepatitis E virus ORF3 protein PxxP motif with heterologous late domain motifs affects virus release via interaction with TSG101.
Article in Virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Bioinformatics Analysis of the Key lncRNAs of the Viral Response Pathway in HepG2 Expressing Genotype IV Swine Hepatitis E Virus ORF3.Microorganisms · 2025Article
- Cell line-dependent release of quasi-enveloped hepatitis E virus reveals alternative Golgi-associated egress in the absence of pORF3.Frontiers in cellular and infection microbiology · 2025Article
- Antagonism of epidermal growth factor receptor signaling favors hepatitis E virus life cycle.Journal of virology · 2024Article
- Morphogenesis of Hepatitis E Virus.Advances in experimental medicine and biology · 2023Article
- Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.Microbiology spectrum · 2022Article
- Identification of Hepatitis E Virus Genotypes 3 and 7 in Israel: A Public Health Concern?Viruses · 2021Article
- Distribution and Pathogenicity of Two Cutthroat Trout Virus (CTV) Genotypes in Canada.Viruses · 2021Article
- Open reading frame 3 protein of hepatitis E virus: Multi-function protein with endless potential.World journal of gastroenterology · 2021Review
- Advances in Hepatitis E Virus Biology and Pathogenesis.Viruses · 2021Review
- Structural and molecular biology of hepatitis E virus.Computational and structural biotechnology journal · 2021Review
- Deep Insight Into Long Non-coding RNA and mRNA Transcriptome Profiling in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3.Frontiers in veterinary science · 2021Article
- Stem Cell-Derived Culture Models of Hepatitis E Virus Infection.Cold Spring Harbor perspectives in medicine · 2019Review
- Evolutionary Origins of Enteric Hepatitis Viruses.Cold Spring Harbor perspectives in medicine · 2018Review
- Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion.PLoS pathogens · 2018Article
- Article
- Hepatitis E virus: advances and challenges.Nature reviews. Gastroenterology & hepatology · 2018Review
- ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication.Journal of virology · 2017Article
- Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Hepatitis E Virus (HEV) egress: Role of BST2 (Tetherin) and interferon induced long non- coding RNA (lncRNA) BISPR.PloS one · 2017Article
- Distinct Entry Mechanisms for Nonenveloped and Quasi-Enveloped Hepatitis E Viruses.Journal of virology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The ORF3 protein of hepatitis E virus (HEV) contains a "PSAP" amino acid late domain motif, which allows for interaction with the endosomal sorting complexes required for transport (ESCRT) pathway aiding virion release. Late domain motifs are interchangeable with other viral late domain motifs in several enveloped viruses, however, it remains unknown whether HEV shares this functional interchangeability and what implications this might have on viral replication. In this study, by substituting heterologous late domain motifs (PPPY, YPDL, and PSAA) for the HEV ORF3 late domain (PSAP), we demonstrated that deviation from the PSAP motif reduces virus release as measured by viral RNA in culture media. Virus release could not be restored by insertion of a heterologous late domain motif or by supplying wild-type ORF3 in trans, suggesting that the HEV PSAP motif is required for viral exit which cannot be bypassed by the use of alternative heterologous late domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.