ArticleBioMed research international2015
A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- A multiplex serological assay for quantification of arbovirus antibody kinetics and neutralizing antibodies.PLoS neglected tropical diseases · 2026Article
- An experimental multiplex PCR/RT-PCR for detection of the main viruses associated with equine neurological disease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Development of an indirect ELISA for the detection of venezuelan equine encephalitis virus specific antibodies in horses.PloS one · 2026Article
- Multiplex serological screening of wild boar as sentinels of emerging zoonoses: HEV, WNV, and TBEV distribution in Saxony, Germany.One health (Amsterdam, Netherlands) · 2025Article
- Epidemiological and antigenic inferences from serological cross-reactivity among arboviruses.Science translational medicine · 2025Article
- Serological differentiation of West Nile, Usutu, and tick-borne encephalitis virus antibodies in birds and horses using mutant E protein ELISAs.Scientific reports · 2025Article
- Meteorological and environmental factors associated with the exposure to tick-borne encephalitis virus (TBEV) in cattle, north-eastern France, 2018-2019.Veterinary research · 2025Article
- Seroprevalence of West Nile, Usutu and tick-borne encephalitis viruses in equids from south-western France in 2023.Veterinary research · 2025Article
- Molecular and Serological Surveillance of Mosquito-Borne Viruses in Racehorses or Mosquitoes From Horse Farms in Shanghai, China, 2022.Transboundary and emerging diseases · 2025Article
- European Preparedness for Japanese Encephalitis Virus Through Alignment of Animal Health Laboratory Diagnosis.Transboundary and emerging diseases · 2025Article
- Lack of Serological and Molecular Evidence of Duck Tembusu Virus Infection in Ducks from South Korea.Veterinary sciences · 2024Article
- First evidence of circulation of multiple arboviruses in Algeria.PLoS neglected tropical diseases · 2024Article
- Autoantibodies neutralizing type I IFNs underlie severe tick-borne encephalitis in ∼10% of patients.The Journal of experimental medicine · 2024Article
- Informing an investment case for Japanese encephalitis vaccine introduction in Bangladesh.Science advances · 2024Article
- Exposure of cattle to tick-borne encephalitis virus in the historical endemic zone in north-eastern France.BMC veterinary research · 2024Article
- Accelerating targeted mosquito control efforts through mobile West Nile virus detection.Parasites & vectors · 2024Article
- Recombinant TBEV Protein E of the Siberian Subtype Is a Candidate Antigen in the ELISA Test System for Differential Diagnosis.Diagnostics (Basel, Switzerland) · 2023Article
- Severe Neurologic Disease in a Horse Caused by Tick-Borne Encephalitis Virus, Austria, 2021.Viruses · 2023Article
- Seroprevalence forEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Article
- West Nile, Sindbis and Usutu Viruses: Evidence of Circulation in Mosquitoes and Horses in Tunisia.Pathogens (Basel, Switzerland) · 2023Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses. The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs). These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members. Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities. The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using the Drosophila S2 expression system. Purified antigens were covalently bonded to fluorescent beads. The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls. rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera. This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.
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