Evidence map›Paper›PMID 26456825›Full record

ArticleCell reports2015

Polar Ejection Forces Promote the Conversion from Lateral to End-on Kinetochore-Microtubule Attachments on Mono-oriented Chromosomes.

Danica Drpic, António J Pereira, Marin Barisic, Thomas J Maresca, Helder Maiato

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Mechanism and regulation of kinesin motors.Nature reviews. Molecular cell biology · 2025
    Review
  3. Review
  4. Article
  5. Principles and dynamics of spindle assembly checkpoint signalling.Nature reviews. Molecular cell biology · 2023
    Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. RZZ-SPINDLY-DYNEIN: you got to keep 'em separated.Cell cycle (Georgetown, Tex.) · 2020
    Review
  16. Article
  17. Review
  18. Article
  19. Chromokinesins.Current biology : CB · 2018
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Danica DrpicChromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; Instituto de Investigação e Inovação em Saúde - i3S, Universidade do Porto, 4099-002 Porto, Portugal; Graduate Program in Areas of Basic and Applied Biology (GABBA), Instituto de Ciencias Biomedicas Abel Salazar da Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal.
António J PereiraChromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; Instituto de Investigação e Inovação em Saúde - i3S, Universidade do Porto, 4099-002 Porto, Portugal.
Marin BarisicChromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; Instituto de Investigação e Inovação em Saúde - i3S, Universidade do Porto, 4099-002 Porto, Portugal.
Thomas J MarescaBiology Department, University of Massachusetts, Amherst, Amherst, MA 01003, USA.
Helder MaiatoChromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; Instituto de Investigação e Inovação em Saúde - i3S, Universidade do Porto, 4099-002 Porto, Portugal; Cell Division Unit, Department of Experimental Biology, Faculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal. Electronic address: maiato@ibmc.up.pt.

Funding

Mechano-molecular regulation of kinetochore functionR01GM107026 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI MARESCA, THOMAS JOSEPH · 2013 to 2022
$2.7M
European Research Council 260892NIGMS NIH HHS 5 R01 GM107026NIGMS NIH HHS R01 GM107026
6 · The paper itself

Abstract

Chromosome bi-orientation occurs after conversion of initial lateral attachments between kinetochores and spindle microtubules into stable end-on attachments near the cell equator. After bi-orientation, chromosomes experience tension from spindle forces, which plays a key role in the stabilization of correct kinetochore-microtubule attachments. However, how end-on kinetochore-microtubule attachments are first stabilized in the absence of tension remains a key unanswered question. To address this, we generated Drosophila S2 cells undergoing mitosis with unreplicated genomes (SMUGs). SMUGs retained single condensed chromatids that attached laterally to spindle microtubules. Over time, laterally attached kinetochores converted into end-on attachments and experienced intra-kinetochore stretch/structural deformation, and SMUGs eventually exited a delayed mitosis with mono-oriented chromosomes after satisfying the spindle-assembly checkpoint (SAC). Polar ejection forces (PEFs) generated by Chromokinesins promoted the conversion from lateral to end-on kinetochore-microtubule attachments that satisfied the SAC in SMUGs. Thus, PEFs convert lateral to stable end-on kinetochore-microtubule attachments, independently of chromosome bi-orientation.

Indexed as

AnimalsBiomechanical PhenomenaCell LineChromosomes, InsectDrosophilaKinesinsKinetochoresMicrotubulesMitosisProtein BindingSpindle ApparatusKinesinschromokinesinskinetochoremitosismitosis with unreplicated genomesspindle assembly checkpoint

Identifiers

PMID26456825
PMCPMC4623360

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.