ArticleCell reports2015
Yeast Replicator: A High-Throughput Multiplexed Microfluidics Platform for Automated Measurements of Single-Cell Aging.
Article in Cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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Who cites it
40 citing papers in PubMed, 82 citations in OpenAlex.
- BudFinder: A Masked Auto-Encoder vision transformer framework for yeast budding detection and lifespan quantification.PLoS computational biology · 2026Article
- Fine-tuning the yeast GAL10 promoter and growth conditions for efficient recombinant membrane protein production and purification.Protein science : a publication of the Protein Society · 2025Article
- A mortality timer based on nucleolar size triggers nucleolar integrity loss and catastrophic genomic instability.Nature aging · 2024Article
- Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip.Biology open · 2024Article
- Capturing of extracellular vesicles derived from single cells ofLab on a chip · 2024Article
- Sis2 regulates yeast replicative lifespan in a dose-dependent manner.Nature communications · 2023Article
- Dietary change without caloric restriction maintains a youthful profile in ageing yeast.PLoS biology · 2023Article
- Time-resolved microfluidics unravels individual cellular fates during double-strand break repair.BMC biology · 2022Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- DetecDiv, a generalist deep-learning platform for automated cell division tracking and survival analysis.eLife · 2022Article
- The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae.Biogerontology · 2022Review
- A high-throughput microfluidic diploid yeast long-term culturing (DYLC) chip capable of bud reorientation and concerted daughter dissection for replicative lifespan determination.Journal of nanobiotechnology · 2022Article
- Modeling aging and its impact on cellular function and organismal behavior.Experimental gerontology · 2021Review
- Inactivating histone deacetylase HDA promotes longevity by mobilizing trehalose metabolism.Nature communications · 2021Article
- Real-Time Monitoring of Dissection Events of Single Budding Yeast in a Microfluidic Cell-Culturing Device Integrated With Electrical Impedance Biosensor.Frontiers in bioengineering and biotechnology · 2021Article
- Understanding the Impact of Industrial Stress Conditions on Replicative Aging inFrontiers in fungal biology · 2021Review
- Slipstreaming Mother Machine: A Microfluidic Device for Single-Cell Dynamic Imaging of Yeast.Micromachines · 2020Article
- Characterization of the impact of GMP/GDP synthesis inhibition on replicative lifespan extension in yeast.Current genetics · 2020Article
- Trajectories of Aging: How Systems Biology in Yeast Can Illuminate Mechanisms of Personalized Aging.Proteomics · 2020Review
- Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.Molecular systems biology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The yeast Saccharomyces cerevisiae is a model organism for replicative aging studies; however, conventional lifespan measurement platforms have several limitations. Here, we present a microfluidics platform that facilitates simultaneous lifespan and gene expression measurements of aging yeast cells. Our multiplexed high-throughput platform offers the capability to perform independent lifespan experiments using different yeast strains or growth media. Using this platform in minimal media environments containing glucose, we measured the full lifespan of individual yeast cells in wild-type and canonical gene deletion backgrounds. Compared to glucose, in galactose we observed a 16.8% decrease in replicative lifespan accompanied by an ∼2-fold increase in single-cell oxidative stress levels reported by PSOD1-mCherry. Using PGAL1-YFP to measure the activity of the bistable galactose network, we saw that OFF and ON cells are similar in their lifespan. Our work shows that aging cells are committed to a single phenotypic state throughout their lifespan.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.