ArticleChembiochem : a European journal of chemical biology2015
A Comparative Analysis of Acyl-Homoserine Lactone Synthase Assays.
Article in Chembiochem : a European journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- In vitro synthesis and biochemical characterization of acyl-homoserine lactone synthase and its deletion mutant.PloS one · 2024Article
- Article
- Insights into β-ketoacyl-chain recognition for β-ketoacyl-ACP utilizing AHL synthases.Chemical communications (Cambridge, England) · 2018Article
- Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Chemical probes of quorum sensing: from compound development to biological discovery.FEMS microbiology reviews · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Quorum sensing is cell-to-cell communication that allows bacteria to coordinate attacks on their hosts by inducing virulent gene expression, biofilm production, and other cellular functions, including antibiotic resistance. AHL synthase enzymes synthesize N-acyl-l-homoserine lactones, commonly referred to as autoinducers, to facilitate quorum sensing in Gram-negative bacteria. Studying the synthases, however, has proven to be a difficult road. Two assays, including a radiolabeled assay and a colorimetric (DCPIP) assay are well-documented in literature to study AHL synthases. In this paper, we describe additional methods that include an HPLC-based, C-S bond cleavage and coupled assays to investigate this class of enzymes. In addition, we compare and contrast each assay for both acyl-CoA- and acyl-ACP-utilizing synthases. The expanded toolkit described in this study should facilitate mechanistic studies on quorum sensing signal synthases and expedite discovery of antivirulent compounds.
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Registered trials
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