Evidence map›Paper›PMID 26456319›Full record

ArticleJournal of cellular biochemistry2016

The Dmp1-SOST Transgene Interacts With and Downregulates the Dmp1-Cre Transgene and the Rosa(Notch) Allele.

Stefano Zanotti, Ernesto Canalis

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Stefano ZanottiDepartments of Orthopaedic Surgery and Medicine, and The UConn Musculoskeletal Institute, UConn Health, Farmington, 06030, Connecticut.
Ernesto CanalisDepartments of Orthopaedic Surgery and Medicine, and The UConn Musculoskeletal Institute, UConn Health, Farmington, 06030, Connecticut.
UConn Health · US

Funding

Role of Notch Signaling in OsteocytesR01AR063049 · NIAMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI CANALIS, ERNESTO · 2013 to 2017
$1.8M
NIAMS NIH HHS AR063049NIAMS NIH HHS R01 AR063049
6 · The paper itself

Abstract

Activation of Notch1 in osteocytes of Rosa(Notch) mice, where a loxP-flanked STOP cassette and the Nicd coding sequence were targeted to the reverse orientation splice acceptor (Rosa)26 locus, causes osteopetrosis associated with suppressed Sost expression and enhanced Wnt signaling. To determine whether Sost downregulation mediates the effects of Notch activation in osteocytes, Rosa(Notch) mice were crossed with transgenics expressing Cre recombinase or SOST under the control of the dentin matrix protein (Dmp)1 promoter. Dmp1-SOST transgenics displayed vertebral osteopenia and a modest femoral cancellous and cortical bone phenotype, whereas hemizygous Dmp1-Cre transgenics heterozygous for the Rosa(Notch) allele (Dmp1-Cre;Rosa(Notch)) exhibited osteopetrosis. The phenotype of Notch activation in osteocytes was prevented in Dmp1-Cre;Rosa(Notch) mice hemizygous for the Dmp1-SOST transgene. The effect was associated with downregulated Notch signaling and suppressed Dmp1 and Rosa26 expression. To test whether SOST regulates Notch expression in osteocytes, cortical bone cultures from Dmp1-Cre;Rosa(Notch) mice or from Rosa(Notch) control littermates were exposed to recombinant human SOST. The addition of SOST had only modest effects on Notch target gene mRNA levels and suppressed Dmp1, but not Cre or Rosa26, expression. These findings suggest that prevention of the Dmp1-Cre;Rosa(Notch) skeletal phenotype by Dmp1-SOST is not secondary to SOST expression but to interactions among the Dmp1-SOST and Dmp1-Cre transgenes and the Rosa26 locus. In conclusion, the Dmp1-SOST transgene suppresses the expression of the Dmp1-Cre transgene and of Rosa26.

Indexed as

Adaptor Proteins, Signal TransducingAllelesAnimalsBone Diseases, MetabolicExtracellular Matrix ProteinsFemurGene ExpressionGlycoproteinsIntegrasesIntercellular Signaling Peptides and ProteinsMice, Inbred C57BLMice, TransgenicOsteocytesOsteopetrosisProtein BindingReceptor, Notch1Adaptor Proteins, Signal TransducingCre recombinaseDmp1 protein, mouseExtracellular Matrix ProteinsGlycoproteinsGt(ROSA)26Sor non-coding RNA, mouseIntegrasesIntercellular Signaling Peptides and ProteinsReceptor, Notch1RNA, UntranslatedSost protein, mouseBONE REMODELINGCre RECOMBINASEDmp1OSTEOCYTESROSA26 LOCUSSOST

Identifiers

PMID26456319
PMCPMC4777692
OpenAlexW1901457897

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.