ArticleLab on a chip2015
Phenotypic drug profiling in droplet microfluidics for better targeting of drug-resistant tumors.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Gradient-driven microfluidic framework for the screening of ratiometric drug combinations towards precision oncology in paediatric leukaemia.Nature biomedical engineering · 2026Article
- SERSµDrop: A Platform to Study Cell-Cell Communication via SERS Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Review
- Single Cell Droplet-Based Efficacy and Transcriptomic Analysis of a Novel Anti-KLRG1 Antibody for Elimination of Autoreactive T Cells.Research square · 2024Article
- An innovative single-cell approach for phenotyping and functional genotyping of CAR NK cells.Journal for immunotherapy of cancer · 2024Article
- On the synergy of biomicrofluidic technologies and real-time 3D tracking: A perspective.Biomicrofluidics · 2023Article
- Interface Dynamics and the Influence of Gravity on Droplet Generation in a Y-microchannel.Micromachines · 2022Article
- Review
- Droplet microfluidics for functional temporal analysis and cell recovery on demand using microvalves: application in immunotherapies for cancer.Lab on a chip · 2022Article
- CancerCellTracker: a brightfield time-lapse microscopy framework for cancer drug sensitivity estimation.Bioinformatics (Oxford, England) · 2022Article
- Application of microfluidic chips in anticancer drug screening.Bosnian journal of basic medical sciences · 2022Review
- Machine learning phenomics (MLP) combining deep learning with time-lapse-microscopy for monitoring colorectal adenocarcinoma cells gene expression and drug-response.Scientific reports · 2022Article
- Application of Microfluidic Systems for Breast Cancer Research.Micromachines · 2022Review
- Microfluidic technology for multiple single-cell capture.Biomicrofluidics · 2021Review
- Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Investigation of Different Free Image Analysis Software for High-Throughput Droplet Detection.ACS omega · 2021Article
- Microengineered 3D Tumor Models for Anti-Cancer Drug Discovery in Female-Related Cancers.Annals of biomedical engineering · 2021Review
- Phenotype-related drug sensitivity analysis of single CTCs for medicine evaluation.Chemical science · 2020Article
- Machine learning-aided quantification of antibody-based cancer immunotherapy by natural killer cells in microfluidic droplets.Lab on a chip · 2020Article
- Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules.Nature communications · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Acquired drug resistance is a key factor in the failure of chemotherapy. Due to intratumoral heterogeneity, cancer cells depict variations in intracellular drug uptake and efflux at the single cell level, which may not be detectable in bulk assays. In this study we present a droplet microfluidics-based approach to assess the dynamics of drug uptake, efflux and cytotoxicity in drug-sensitive and drug-resistant breast cancer cells. An integrated droplet generation and docking microarray was utilized to encapsulate single cells as well as homotypic cell aggregates. Drug-sensitive cells showed greater death in the presence or absence of Doxorubicin (Dox) compared to the drug-resistant cells. We observed heterogeneous Dox uptake in individual drug-sensitive cells while the drug-resistant cells showed uniformly low uptake and retention. Dox-resistant cells were classified into distinct subsets based on their efflux properties. Cells that showed longer retention of extracellular reagents also demonstrated maximal death. We further observed homotypic fusion of both cell types in droplets, which resulted in increased cell survival in the presence of high doses of Dox. Our results establish the applicability of this microfluidic platform for quantitative drug screening in single cells and multicellular interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.